Anti -TBP antibody, Mouse monoclonal
SIGMA/T1827 - clone 58C9, purified from hybridoma cell culture
Synonym: Anti-GTF2D; Anti-GTF2DI; Anti-SCA17; Anti-TATA box binding protein; Anti-TFIID
MDL Number: MFCD01322691
Product Type: Chemical
Western Blotting JOURNAL CITATION: Effects on murine behavior and lifespan of selectively decreasing expression of mutant huntingtin allele by supt4h knockdown. By: Cheng, H. M., Chern, Y., et al. in PLoS Genet, 2015. PubMed ID: 25760041 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1005043), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Equal amount of Nuclear cell extract of D.Mel cells were incubated with a serial concentration of Monoclonal Anti-TBP (Cat. No. T1827). The antibody was developed with Goat Anti-Mouse – Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1: 2 μg/mL antibody 2: 1 μg/mL antibody 3: 0.5 μg/mL antibody 4: 0.2 μg/mL antibody 5: Negative control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 58C9 |
| conjugate | unconjugated |
| isotype | IgG2b |
| mol wt | antigen ~42 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | Sf9 cell line, human, yeast, Drosophila melanogaster |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 1-2 μg/mL using nuclear extracts of D.Mel cells | |
| UniProt accession no. | P20226  |
| Application: | Monoclonal Anti-TBP antibody produced in mouse has been used in: • immunoblotting • co-immunoprecipitation • immunohistochemistry |
| Biochem/physiol Actions: | TBP facilitates promoter recognition with the help of the sequence-specific binding of the TATA element found in many promoters. |
| Biochem/physiol Actions: | The TBP (TATA-binding protein) gene encodes a subunit of TFIIIB (transcription factor IIIB) complex and recognizes the TATA box in the DNA. Thus, it plays an important role in RNA polymerase II transcription machinery. TBP is known to induce stress granule formation in hepatitis B viral infection. Mutation in the gene is associated with the development of spinocerebellar ataxia 17 (SCA17). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-TBP (mouse IgG2b isotype) is derived from the hybridoma 58C9 produced by the fusion of mouse myeloma cells and splenocytes from Swiss Webster mice immunized with Drosophila TFIID complex. Transcription factor IID (TFIID) complex contains the TATA-binding protein (TBP) and over a dozen of TBP-associated factors (TAFs). |
| General description: | TBP (TATA-binding protein) is encoded by the gene mapped to human chromosome 6q27. The encoded protein is a key constituent of RNA polymerase II transcription apparatus in eukaryotic cells. |
| General description: | TBP encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of tr |
| Immunogen: | Drosophila TFIID complex. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |