Thymidine
SIGMA/T1895 - powder, BioReagent, suitable for cell culture
Synonym: 1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil; 1-(2-Deoxy-β-D-ribofuranosyl)thymine; 2′-Deoxythymidine; Thymine deoxyriboside; dT
CAS Number: 50-89-5
Empirical Formula (Hill Notation): C10H14N2O5
Molecular Weight: 242.23
EC Number: 200-070-4
MDL Number: MFCD00006537
Linear Formula: C10H14N2O5
Product Type: Chemical
assay | ≥99% |
biological source | synthetic (organic) |
form | powder |
InChI | 1S/C10H14N2O5/c1-5-3-12(1 |
InChI key | IQFYYKKMVGJFEH-XLPZGREQSA |
mp | 186-188 °C (lit.) |
product line | BioReagent |
Quality Level | 200 ![]() |
SMILES string | CC1=CN([C@H]2C[C@H](O)[C@ |
solubility | water: 50 mg/mL, clear, colorless |
technique(s) | cell culture | mammalian: suitable |
Application: | Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used: • as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts • as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies • to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells |
Biochem/physiol Actions: | Thymidine is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine. |
General description: | Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine. |
Packaging: | 1, 5, 10, 25 g in poly bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99% |
mp | 186-188 °C (lit.) |
UNSPSC | 12352200 |