Thymidine
SIGMA/T1895 - powder, BioReagent, suitable for cell culture
Synonym: 1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil; 1-(2-Deoxy-β-D-ribofuranosyl)thymine; 2′-Deoxythymidine; Thymine deoxyriboside; dT
CAS Number: 50-89-5
Empirical Formula (Hill Notation): C10H14N2O5
Molecular Weight: 242.23
EC Number: 200-070-4
MDL Number: MFCD00006537
Linear Formula: C10H14N2O5
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T1895-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T1895-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T1895-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T1895-10G
| assay | ≥99% |
| biological source | synthetic (organic) |
| form | powder |
| InChI | 1S/C10H14N2O5/c1-5-3-12(1 |
| InChI key | IQFYYKKMVGJFEH-XLPZGREQSA |
| mp | 186-188 °C (lit.) |
| product line | BioReagent |
| Quality Level | 300  |
| SMILES string | CC1=CN([C@H]2C[C@H](O)[C@ |
| solubility | water: 50 mg/mL, clear, colorless |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Thymidine can be used in HT and HAT cocktails for hybridoma fusion, selection and cloning. It has been used: • as a nucleotide source in minimal essential medium to evaluate the outgrowth of the iSCNT-derived blastocysts • as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) selection medium used for the production of anti-neurogranin (NRGN) antibodies • to prepare THMG (thymidine, hypoxanthine, glycine, methotrexate) and THG stock solution for cleansing mouse lymphoma cells |
| Biochem/physiol Actions: | Thymidine is useful in cell synchronization during S-phase. In the salvage pathway of pyrimidines, pyrimidine phosphorylase reversibly converts thymine to thymidine. |
| General description: | Thymidine is also referred to as pyrimidine deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double stranded structure, thymidine pairs with deoxyadeninosine. |
| Packaging: | 1, 5, 10, 25 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% |
| mp | 186-188 °C (lit.) |
| UNSPSC | 12352207 |