Anti-TRF1 antibody, Mouse monoclonal
SIGMA/T1948 - ~2 mg/mL, clone TRF-78, purified from hybridoma cell culture
Synonym: Anti-Telomeric Repeat Binding Factor 1
MDL Number: MFCD03792434
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 10 μg/mL Monoclonal Anti-TRF1 Clone: TRF-78 (Cat. No. T1948). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Monoclonal Anti-TRF1 Clone: TRF-78 (Cat. No. T1948). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa Nuclear 2. HeLa 3. HEK-293T 4. U2OS 5. HepG2 6. P19 7. PC12
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TRF-78, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~70 kDa by SDS-PAGE |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: suitable |
| microarray: suitable | |
| western blot: 2-4 μg/mL using HeLa nuclear extract | |
| UniProt accession no. | P54274  |
| Application: | Monoclonal Anti-TRF1 antibody produced in mouse has been used in Western blotting and enzyme linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | TRF1 (TTAGGG repeat binding factor 1) has a negative effect on the length of the telomer. Overexpression of TRF1 in cancer cells that contain telomerase activity, causes the shortening of the length of their telomers. While inhibition of TRF1 causes the elongation of telomers. It was shown that the level of TRF1 in the cells does not affect the expression of the telomerase protein. This suggests that TRF1 may act directly on the activity of the telomerase protein. Tankyrase is a protein that interacts with TRF1 and its C-terminal region is homologous to poly (adenosine dinucleotide phosphate (ADP)-ribose) polymerase (PARP). In response to DNA damage, the PARP protein mediates ADP-ribose polymers of protein acceptors. In vitro studies have shown that tankyrase is responsible for that polyribosylation of TRF1, which in turn abolishes its ability to bind telomers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-TRF1 (mouse IgG1 isotype) is derived from the TRF-78 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a human TRF1 protein produced in baculovirus. TRF1 and TRF2 (TTAGGG repeat binding factors) are two major proteins that bind to human telomers. TRF1 has a DNA binding domain with high homology to the Myb family of transcription factors. Unlike the Myb family that contains only one DNA binding motif, TRF1 has multiple of this motif. |
| Immunogen: | human TRF1 protein produced in baculovirus. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| Specificity: | Monoclonal Anti-TRF1 recognizes human TRF1. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |