Trichostatin A, Ready Made Solution
SIGMA/T1952 - 5 mM in DMSO, from Streptomyces sp.
Synonym: TSA; [R-
CAS Number: 58880-19-6
Empirical Formula (Hill Notation): C17H22N2O3
Molecular Weight: 302.37
Linear Formula: C17H22N2O3
Product Type: Chemical
| antibiotic activity spectrum | fungi |
| neoplastics | |
| assay | ≥98% (HPLC) |
| biological source | Streptomyces sp. |
| concentration | 5 mM in DMSO |
| form | DMSO solution |
| InChI | 1S/C17H22N2O3/c1-12(5-10- |
| InChI key | RTKIYFITIVXBLE-WKWSCTOISA |
| mode of action | enzyme | inhibits |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Trichostatin A, ready-made solution has been used: • as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing • to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH) • to treat cells for the HDAC inhibition experiments |
| Biochem/physiol Actions: | Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning. |
| General description: | Trichostatin A is a compound of primary hydroxamic acid. |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |