Trichostatin A, Ready Made Solution
SIGMA/T1952 - 5 mM in DMSO, from Streptomyces sp.
Synonym: TSA; [R-
CAS Number: 58880-19-6
Empirical Formula (Hill Notation): C17H22N2O3
Molecular Weight: 302.37
Linear Formula: C17H22N2O3
Product Type: Chemical
antibiotic activity spectrum | fungi |
neoplastics | |
assay | ≥98% (HPLC) |
biological source | Streptomyces sp. |
concentration | 5 mM in DMSO |
form | DMSO solution |
InChI | 1S/C17H22N2O3/c1-12(5-10- |
InChI key | RTKIYFITIVXBLE-WKWSCTOISA |
mode of action | enzyme | inhibits |
Quality Level | 200 ![]() |
shipped in | dry ice |
storage temp. | −20°C |
technique(s) | cell culture | mammalian: suitable |
Application: | Trichostatin A, ready-made solution has been used: • as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing • to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH) • to treat cells for the HDAC inhibition experiments |
Biochem/physiol Actions: | Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning. |
General description: | Trichostatin A is a compound of primary hydroxamic acid. |
RIDADR | NA 1993 / PGIII |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (HPLC) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |