apo-Transferrin human
SIGMA/T2036 - powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)
Synonym: Human transferrin; Siderophilin
CAS Number: 11096-37-0
EC Number: 234-318-8
MDL Number: MFCD00130536
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T2036-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T2036-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T2036-500MG
| assay | ≥98% (agarose gel electrophoresis) |
| cation traces | Fe: ≤0.005% |
| concentration | ~25 mM |
| form | powder |
| impurities | endotoxin, tested |
| HIV and HBsAg, source material tested negative | |
| mol wt | 76-81 kDa |
| product line | BioReagent |
| Quality Level | 200  |
| shipped in | ambient |
| solubility | H2O: 50 mg/mL |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02787  |
| Application: | apo-Transferrin human has been used:• to culture human primary pancreatic endothelial cells, Het1As (non-tumorous esophagus cells), and immortalized human colonic epithelial cells (HCEC-1CT) • to culture α mouse liver 12 (AML-12) (mature hepatocytes) cells • as a source for human apo-transferrin for purification before crystallography |
| Biochem/physiol Actions: | Transferrin is responsible for transporting iron from the sites of absorption and storage to the tissue cells. It maintains the levels of iron in biological fluids. The levels of transferrin may indicate the total iron-binding capacity (TIBC). Transferrin supplies the required iron for incorporation into hemoglobin within RBCs in the bone marrow. Iron deficiency causes an increase in the levels of transferrin. Pregnancy and estrogen administration can also raise the levels of transferrin. Chronic liver disease, renal insufficiency, malnutrition, and protein-losing enteropathies reduce the synthesis of transferrin. |
| General description: | Transferrin, majorly synthesized in the liver, is an abundant glycoprotein in the serum. The members of the transferrin superfamily have similar polypeptide folding patterns. Transferrin contains N- and C-terminal iron-binding homologous domains. Each of these domains is split into two subdomains having binding sites for iron and anions within the inter-subdomain cleft. The binding cleft opens and closes with iron releasing and iron binding. apo-Transferrin is an iron-free protein that arises after transferrin dissociates from its receptor. This product can be supplemented with iron or used to bind free iron present in media. |
| Other Notes: | Non-heme iron-transport protein. |
| Packaging: | 1 g in poly bottle |
| Packaging: | 100, 500 mg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Purity | ≥98% (agarose gel electrophoresis) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |