Anti-β-Tubulin III antibody produced in rabbit
SIGMA/T2200 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Tuj 1
MDL Number: MFCD01323870
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Regeneration of neural crest derivatives in the Xenopus tadpole tail. By: Lin, G., Chen, Y., et al. in BMC Dev Biol, 2007. PubMed ID: 17521450 Image collected and cropped by CiteAb from the following publication, (http://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-7-56), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Regeneration of neural crest derivatives in the Xenopus tadpole tail. By: Lin, G., Chen, Y., et al. in BMC Dev Biol, 2007. PubMed ID: 17521450 Image collected and cropped by CiteAb from the following publication, (http://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-7-56), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Genetic Strategies HeLa TUBB3 KO (knocked out ) Cell Lysate vs. HeLa wild type Cell Lysate were separated on SDS-PAGE probed with 1:3,000 Anti-β-Tubulin III antibody produced in Rabbit (Cat. No. T2200). Anti-GAPDH antibody, Mouse monoclonal, clone: GAPDH-71.1 (Cat. No. G8795) was used as loading control. The antibodies were developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545), Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa TUBB3 KO (knocked out) 2. HeLa wild type Results No antibody staining can be indicated in TUBB3 KO (knocked out) lysate as the intended target has been genetically removed lane (1) and the corresponding wild type sample demonstrate recognition of the target lane (2). Equal staining of the GAPDH protein confirm the loading control equal amount of the lysates.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human brain and cerebellum sections with 10 μg/mL Rabbit Anti-β-Tubulin III (Cat. No. T2200) using Biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence : SCK-CEN Mouse cortex primary neurons cultured for 10 days were stained for PSD95, Β-Tubulin III and nuclei. For PSD95 Monoclonal Anti-PSD95 antibody produced in Mouse (Cat. No. P246) and Anti-Mouse IgG (whole molecule)–FITC antibody produced in Goat (Cat. No. F2012) (green) were used. For Β-Tubulin III Anti-Β-Tubulin III antibody produced in Rabbit (Cat. No. T2200) and Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in Goat (Cat. No. T6778) (red). Nuclei were stained with Hoechst B2883 (blue/purple). Submitted by Dr G. Pani, Studiecentrum voor Kernenergie-Centre d′Etude d′Energie Nucleaire
Immunofluorescence SH-SY-5Y cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 2 μg/mL Rabbit Anti-β-Tubulin III (Cat. No. T2200). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 0.4 μg/mL Rabbit Anti-β-Tubulin III (Cat. No. T2200). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa 2. U87 3. COS7 4. P19 5. MDBK
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | knockout Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~55 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): 10 μg using RIPA extract (250 μg) of cultured rat PC12 cells |
| indirect immunofluorescence: 10-20 μg/mL using rat PC12 cells | |
| western blot: 0.2-0.4 μg/mL using whole extracts of mouse brain or cultured human neuroblastoma SH-SY5Y cells | |
| UniProt accession no. | Q13509  |
| Application: | Anti-β-Tubulin III antibody produced in rabbit has been used in following studies: • Immunofluorescence • Immunostaining • Western blotting |
| Application: | Anti-β-Tubulin III antibody produced in rabbit has been used in following studies: • immunofluorescence • immunostaining • western blotting • indirect immunoperoxidase assay (IPA) |
| Biochem/physiol Actions: | Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons. α/β-Tubulin, an integral component of microtubules, is present in almost all eukaryotic cells. α/β-Tubulin occurs mostly as soluble (approx. 100-110 kDa) heterodimeric sets of α- and β-tubulin isotypes or as polymers in assembled microtubules. β-Tubulin III (also designated β-4 chain) is found in the brain and dorsal root ganglia and appears to be localized to neurons of the central and peripheral nervous system. β-tubulin III is also found in Sertoli cells of the testis, spermatozoa tails, certain lung cells, and apparently some breast stromal cells. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 441-450 of human β-tubulin III (Ala446 to Ser446 substitution) with N-terminal added cysteine, conjugated to KLH. The sequence is conserved in mammals. |
| Other Notes: | In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working concentrations by titration. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Preparation Note: | Storage and Stability: For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| Specificity: | Anti-β-Tubulin III recognizes human, mouse,and rat β-tubulin III. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |