Anti-Tumor Necrosis Factor Soluble Receptor Type II antibody produced in goat
SIGMA/T2315 - IgG fraction of antiserum, lyophilized powder
Synonym: CD120b antigen; Etanercept; TNF sRII; TNF-R2; p75 TNF receptor; p75TBPII; p75TNFR; tumor necrosis factor receptor superfamily member 1B
MDL Number: MFCD01633483
Product Type: Chemical
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: 5-15 μg/mL (detects TNF RII in human PBLs) |
| western blot: 1 μg/mL (detects rhs TNF RII at 5 ng/lane under non-reducing conditions) | |
| UniProt accession no. | P20333  |
| Application: | Western Blot: 1 ug/mL detects rhs TNF RII at 5 ng/lane under non-reducing conditions Immunocytochemistry: Concentration of 5-15 ug/ml detects TNF RII in human peripheral blood lymphocytes Optimal dilutions should be determined by the end user. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a highaffinity receptor for TNF alpha and TNF beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist. |
| Immunogen: | recombinant human TNF soluble receptor II (TNF sRII) expressed in Escherichia coli. The immunogen is the non-glycosylated, N-terminal methionyl form of the naturally-occurring human soluble type II receptor for TNF, minus 53 amino acids from the proline-rich region just exterior to the transmembrane domain. |
| Other Notes: | Protein A or G purified |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (pH 7.4) and 5% trehalose. |
| Specificity: | The antibody detects human TNF sRII and shows no cross-reactivity with recombinant human TNF sRI. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |