Trypsin Inhibitor from Glycine max (soybean)
SIGMA/T2327 - BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)
Synonym: Kunitz Inhibitor
CAS Number: 9035-81-8
EC Number: 232-906-9
MDL Number: MFCD00082102
Product Type: Chemical
Trypsin Inhibitor (Kunitz) (soybean)
SDS-PAGE (4-20%) of Trypsin Inhibitor (Cat. Nos. T9128, T9777, and T2327) under reducing conditions and stained with Coomassie blue.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T2327-25MG
| assay | ≥95% (Kunitz inhibitor, SDS-PAGE) |
| biological source | Glycine max (soybean) |
| form | lyophilized powder |
| product line | BioUltra |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Analysis Note: | One mg will inhibit ≥1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein. |
| Application: | Trypsin Inhibitor from Glycine max (soybean) has been used: • as a standard protein to measure the amount of endogenous trypsin inhibitor present in midgut lysate (M1) of Riptortus pedestris • as a standard to compare the trypsin inhibitory activity of the purified protein • to monitor the trypsin inhibitory activity by fractionating in MonoS cation exchange chromatography • as an trypsin inhibitor |
| Biochem/physiol Actions: | Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108. |
| Biochem/physiol Actions: | Trypsin Inhibitor from Glycine max (soybean) binds with the active site of trypsin enzyme, in a competitive inhibition manner. |
| General description: | Trypsin Inhibitor from Glycine max (soybean) also known as Kunitz trypsin inhibitor is a 21 kDa protein with a single trypsin binding reactive site. |
| Other Notes: | View more information on Trypsin Inhibitor . |
| Packaging: | 5, 10, 25 mg in glass bottle |
| Preparation Note: | Further purification of T9128 yielding an electrophoretically pure Kunitz inhibitor with increased activity. |
| Preparation Note: | Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color. |
| Unit Definition: | One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Purity | ≥95% (Kunitz inhibitor, SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |