Tropomyosin from porcine muscle
SIGMA/T2400 - lyophilized powder (contains Tris buffer salts)
CAS Number: 9067-56-5
MDL Number: MFCD00132526
Product Type: Chemical
| assay | ≥70% protein basis (biuret) |
| biological source | Porcine muscle |
| form | lyophilized powder (contains Tris buffer salts) |
| Quality Level | 200  |
| storage temp. | −20°C |
| technique(s) | electrophoresis: suitable |
| Analysis Note: | SDS electrophoresis shows two major bands. |
| Application: | Tropomyosin from porcine muscle has been used: • as a positive control in actin- α-tropomyosin (TPM1) co-sedimentation analysis and assay • in analyzing the anti-adhesive properties of fish tropomyosins • in far Western blot analysis |
| Biochem/physiol Actions: | Tropomyosin is associated with the actin cytoskeleton and plays a role in stabilizing and regulating the interactions of actin with other actin-binding proteins. It also plays a role in muscle contraction by regulating the interactions of actin with myosin. |
| Biochem/physiol Actions: | Tropomyosin together with troponin, regulates the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Various tropomyosin isoforms help confer structure and function to actins in the cytoskeleton and striated muscle function. Dimeric protein that assumes an α-helical coiled-coil conformation. In the absence of Ca2+, it inhibits muscle contractility by blocking the myosin binding sites on actin. |
| General description: | Tropomyosin is a member of the actin filament binding protein family. It is a ubiquitous protein present in animals and fungi. Tropomyosin is made up of two α-helical chains arranged as a coiled-coil. It exists over twenty isoforms, in different eukaryotic cells like skeletal, cardiac, and smooth muscles, brain, platelets, fibroblasts, platelets, and other non-muscle cells. |
| Packaging: | 1 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% protein basis (biuret) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |