Anti-Mouse IgG (whole molecule)−TRITC antibody produced in rabbit
SIGMA/T2402 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Rabbit Anti-Mouse IgG (whole molecule)
MDL Number: MFCD00162646
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:400 Anti-Mouse IgG (whole molecule)-TRITC antibody produced in Rabbit (Cat. No. T2402).
Immunofluorescence Human Periferal Blood Lymphocytes probed and developed using 1:50 Monoclonal Anti-β2-Microglobulin antibody produced in Mouse, Clone: BM-63 (Cat. No. M7398) followed by 1:200 Anti-Mouse IgG (whole molecule)-TRITC antibody produced in Rabbit (Cat. No. T2402).
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | TRITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | direct immunofluorescence: 1:64 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 |
| Application: | Anti-Mouse IgG (whole molecule)-TRITC antibody is suitable for use in direct immunofluorescence (1:64), immunohistochemistry (1:200 dilution in formalin-fixed, paraffin-embedded sections), Ouchterlony double diffusion and electrophoresis techniques. |
| Biochem/physiol Actions: | Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallizable (fc), comprises the H chain constant region. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4. |
| Immunogen: | Normal mouse serum |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Anti-Mouse IgG (whole molecule)-TRITC antibody is specific for mouse IgG. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |