Triosephosphate Isomerase from baker's yeast (S. cerevisiae)
SIGMA/T2507 - Type I, ammonium sulfate suspension, ~10,000 units/mg protein
Synonym: D-
CAS Number: 9023-78-3
EC Number: 232-774-2
MDL Number: MFCD00082080
Product Type: Chemical
| foreign activity | pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphat |
| form | ammonium sulfate suspension |
| mol wt | 53 kDa by equilibrium centrifugation |
| Quality Level | 200  |
| specific activity | ~10,000 units/mg protein |
| storage temp. | 2-8°C |
| type | Type I |
| Analysis Note: | Protein determined by biuret. |
| Application: | Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins. |
| Other Notes: | Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units. |
| Packaging: | 25 mg in glass bottle |
| Physical form: | Crystalline suspension in 2.7 M (NH4)2SO4, 0.5 mM EDTA, pH 6.5 |
| Unit Definition: | One unit will convert 1.0 μmole |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ~10,000 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 5.3.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |