Monoclonal Anti-Tenascin antibody produced in mouse
SIGMA/T2551 - clone BC-24, ascites fluid
Synonym: Anti-Tenascin-N
MDL Number: MFCD00162876
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:4,000 Monoclonal Anti-Tenascin antibody produced in Mouse, Clone: BC-24 (Cat. No. T2551) followed by 20 μg/mL Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B7151) and by 30 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BC-24, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using protease-digested sections of adult human tissue. | |
| immunohistochemistry (frozen sections): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| radioimmunoassay: suitable | |
| western blot: suitable | |
| UniProt accession no. | Q9UQP3  |
| Application: | Monoclonal Anti-Tenascin antibody produced in mouse has been used for immunohistochemistry. |
| Application: | Mouse monoclonal clone BC-24 anti-Tenascin antibody is an essential tool for the localization, identification and studies on the role of tenascin in epithelial-mesenchymal and neuronal-glial interactions. |
| Biochem/physiol Actions: | Tenascin (TN) is up-regulated by epithelial transforming growth factor β (TGF-β) in mesenchyme. TN in the stroma of cancerous tissue contributes to tumorigenesis and progression of metastasis. High molecular weight TN isoform, a stromal marker of breast cancer, over-expresses implicating cell proliferation and invasion. TN has a potential role in tissue restoration. Intracytoplasmic TN immunoreactivity has been detected in malignant melanomas and in lung carcinomas. TN expression, when reacted with monoclonal antibody, determines its localization in tumor cells and its association with epithelial-mesenchymal and neuronal-glial cell interactions. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Human Tenascin (mouse IgG1 isotype) is derived from the BC-24 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| General description: | Tenascin (TN) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. Human TN has 3 subunits of 190, 200 and 220 kD. TN has been independently discovered in a variety of species and tissue types, often in the basement membrane or intercellular spaces. The expression of TN is associated with development and growth, both normal and pathological, with restricted distribution in normal adult tissue. It is synthesized by fibroblasts, chondroblasts, osteocytes, smooth muscle cells and glial cells. Human and chicken TN contain an RGD sequence which may function in cell adhesion and it seems likely that TN mediates cell attachment through an RGD-dependent integrin receptor. Mouse TN does not contain an RGD sequence in the third type III repeat implicated in cell attachment, or in any other positions. |
| Immunogen: | human tenascin. |
| Physical form: | This product is supplied as ascites fluid containing 15 mM sodium azide |
| Specificity: | The antibody recognizes an epitope within the N-terminal EGF-like (EGF-L) sequence of the molecule. It recognizes all human tenascin isoforms. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |