Trizma® hydrochloride solution
SIGMA/T2569 - pH 7.8, BioPerformance Certified, 1 M, suitable for cell culture
Synonym: Tris hydrochloride solution
Product Type: Chemical
absorption | ≤0.05 at 290 at 40% |
concentration | 1 M |
form | solution |
grade | BioPerformance Certified |
for molecular biology | |
impurities | bioburden, tested |
DNase, RNase, Protease, none detected | |
endotoxin, tested | |
≤5 ppm Heavy metals (as Pb) | |
pH | 7.8 |
Quality Level | 200 |
technique(s) | cell culture | mammalian: suitable |
useful pH range | 7.0-9.0 |
Application: | Trizma® hydrochloride solution has been used: • as a component of lysis buffer for lysing human embryonic kidney (HEK) 293 cells • in the 10X digoxigenin (DIG) deoxynucleoside triphosphate (dNTP) nick translation labeling buffer and in tris EDTA and sodium chloride (TEN) buffer • in the preparation of tris, acetate, ethylenediaminetetraaceti |
General description: | A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units. |
General description: | Tris(hydroxymethyl)aminom |
Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
UNSPSC | 12161700 |