Monoclonal Anti-TIS7 antibody produced in mouse

SIGMA/T2576 - ~2 mg/mL, clone IRT537, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Interferon-Related Developmental Regulator 1 (IFRD1) (human); Anti-PC4 (rat); Anti-TPA-Induced Sequence 7 (mouse)

MDL Number: MFCD08705605
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-T2576-200UL	200 µL	$607.00 1/EA	Add To Favorites

Western Blotting Whole extract of COS7 cells transfected with Mouse Tis7 were separated on SDS-PAGE and probed with Monoclonal Anti-TIS7 antibody produced in Mouse, Clone: IRT537 (Cat. No. T2576). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A2179). Lanes 1. 0.25 μg/mL antibody 2. 0.5 μg/mL antibody 3. 1 μg/mL antibody 4. Negative Control (band at 75 kDa- 50 antigen + 25 cMyc)

Properties
Descriptions
Safety Info.
Basic Data

antibody form	purified immunoglobulin
antibody product type	primary antibodies
biological source	mouse
clone	IRT537, monoclonal
concentration	~2 mg/mL
conjugate	unconjugated
form	buffered aqueous solution
isotype	IgG2a
mol wt	antigen ~50 kDa
Quality Level	200
shipped in	dry ice
species reactivity	mouse, human
storage temp.	−20°C
technique(s)	immunocytochemistry: suitable
	indirect ELISA: suitable
	microarray: suitable
	western blot: 0.25-0.5 using extracts of COS7 cells transfected with mouse Tis7
UniProt accession no.	O00458

Application:	Monoclonal Anti-TIS7 antibody produced in mouse has been used in western blot analysis.
Biochem/physiol Actions:	Tetradecanoylphorbol-13-acetate-induced sequence 7 (TIS7)/interferon related developmental regulator 1(FRD1) is implicated in regulation of the proliferation and differentiation pathways induced by nerve growth factor (NGF). The encoded protein also plays a vital role in myocyte differentiation, and it modulates the nuclear factor-κB (NF-κB) pathway by influencing the activity of histone deacetylases and forming complexes with the p65 subunit of NFκB. Genetic variations in the gene is associated with the development of various diseases such as sensory/motor neuropathy with ataxia (SMNA), cystic fibrosis, lung disease and myelodysplastic syndrome (MDS). TIS7 is also implicated in regulation of airway inflammation.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	The tetradecanoylphorbol-13-acetate-induced sequence 7 (TIS7), also known as interferon related developmental regulator 1 (FRD1), is mapped to human chromosome 7q31.1. The gene with 13 exons, spans about 52kb of genomic DNA. The encoded protein consists of 451 amino acids and is expressed in mature neutrophils.
Immunogen:	synthetic peptide corresponding to amino acids 30-47 of mouse Tis7, conjugated to KLH.
Physical form:	Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

WGK Germany

Storage Temp.	−20°C
UNSPSC	12352203

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