Trizma® hydrochloride solution
SIGMA/T2663 - 1 M, BioReagent, for molecular biology
Synonym: Tris hydrochloride solution
MDL Number: MFCD00012590
Product Type: Chemical
| concentration | 1 M |
| form | solution |
| grade | for molecular biology |
| impurities | DNase, RNase, NICKase and protease, none detected |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
| InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
| pH | 7.35-7.45 |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | Cl.NC(CO)(CO)CO |
| sterility | 0.2 μm filtered |
| Application: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Application: | Trizma® hydrochloride along with Trizma-base is used to prepare buffer solutions of pH 7.35. It was used to prepare the triton extract. It may be used in the analysis of protease activity, isolated from the Bacillus stearothermophilus and Bacillus subtilis. It may be employed as buffer for the restriction fragment analysis of genomic DNA isolated from the enterococcal isolates and Escherichia coli. |
| General description: | Trizma® hydrochloride solution is a useful biological buffer. |
| Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12161700 |