Thymidine Phosphorylase, recombinant from Escherichia coli

SIGMA/T2807 - recombinant, expressed in E. coli, buffered aqueous solution, ≥500 units/mL

Synonym: Thymidine:orthophosphate deoxy-D-ribosyltransferase

CAS Number: 9030-23-3
MDL Number: MFCD00132426
Product Type: Chemical

Unit Definition One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.

Application:	Thymidine phosphorylase has been used in a study to assess TAS-102 treatment in patients with advanced solid tumors. Thymidine phosphorylase has also been used in a study to investigate antitumor effects of the FP3 protein on patient-derived tumor tissue xenograft models of primary colon carcinoma.
Biochem/physiol Actions:	An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.
General description:	Thymidine phosphorylase inhibits vascular smooth muscle cell proliferation.
Packaging:	5000 units in glass bottle
Physical form:	Solution in 0.5 M potassium phosphate containing 2 mM uracil, 0.02% sodium azide and bovine serum albumin
Preparation Note:	Cloned from E. coli and produced in overexpressing E. coli
Unit Definition:	One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable