Transforming Growth Factor-β2 human
SIGMA/T2815 - TGF-β2, recombinant, expressed in NSO cells, powder, suitable for cell culture
Synonym: hTGF-β2; TGF-β2
MDL Number: MFCD00163957
Product Type: Chemical
TGF-beta Signaling
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
Research areas: Apoptosis, Differentiation, Epithelial Mesenchymal transition, Cancer biology, Bone remodelling, Developmental biology, Inflammation, Metabolism
Disease areas: Gastrointestinal cancers, Pulmonary, liver and renal fibrosis, Diabetes, Tuberculosis
See also: T7039; H8541; B3555; 11412272001; H4791; H8791
| assay | ≥97% (electrophoresis) |
| biological source | human |
| form | powder |
| impurities | ≤1 EU/μg |
| mol wt | 25 kDa |
| packaging | pkg of 10 μg |
| pkg of 2 μg | |
| potency | 0.04 ng/mL ED50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in NSO cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| suitability | suitable for |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P61812  |
| Analysis Note: | The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells. |
| Application: | Transforming Growth Factor-β2 human has been used in chondrogenic, adipogenic and osteogenic differentiation of mesenchymal stem cells. It has also been used to induce extracellular matrix accumulation in primary human trabecular meshwork cells and further to study the action of tetramethylpyrazine on the same. |
| Biochem/physiol Actions: | TGF-β2 is known to stimulate cell growth, differentiation, migration and survival. TGF-β2 acts as a key regulator for immune homeostasis by controlling lymphocyte proliferation, embryogenesis, hematopoiesis and apoptosis. TGF-β is also associated with inflammation and wound healing and is considered as a strong growth inhibitor for keratinocyte cells. TGF-β is associated with the process of tumor development, progression and metastasis. TGF-β2 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. The TGF-β gene is overexpressed in glioma tumors and also in colon cancer, gastric cancer and cervical lesions. |
| Biochem/physiol Actions: | Transforming growth factor-β2 (TGF-β2), like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets. |
| General description: | The transforming growth factor-β2 (TGFB2) gene is mapped to human chromosome 1q41. The encoded protein is localized to extracellular matrix. TGF-β2 is a member of the TGF-β superfamily. TGF-β exists in three isoforms (TGF-β1, TGF-β2 and TGF-β3) and are known to share similar structure and function. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.1 mg bovine serum albumin |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (electrophoresis) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |