Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster
SIGMA/T2824 - ~2 mg/mL, clone TN3-19.12, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-TNF-α
MDL Number: MFCD01632994
Product Type: Chemical
Western Blotting Whole extract of T7539 recombinant Mouse TNF-α cells were separated on SDS-PAGE and probed with Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in Hamster (Cat. No. T2824). The antibody was developed using 1:10,000 Monoclonal Anti-Hamster IgG-Peroxidase antibody produced in Mouse, Clone: MAH 1.12 (Cat. No. A7851). Lanes 1. 0.25 μg/mL antibody 2. 0.5 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | hamster |
| clone | TN3-19.12, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| neutralization: suitable | |
| western blot: 0.25-0.5 μg/mL using recombinant mouse TNF-α | |
| UniProt accession no. | P16599  |
| Application: | Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster has been used in: • immunoblotting • enzyme-linked immunosorbent assay (ELISA) • immunoprecipitation • neutralization |
| Biochem/physiol Actions: | TNF-α is a major mediator of inflammatory responses and its over-production results in extensive tissue damage associated with endotoxemia and cachexia. High serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections, and various neoplastic diseases. TNF-α exhibits growth-enhancing activities for normal cells like fibroblasts, T and B cells, activation of endothelial cells, and differentiation of myeloid cells. In neoplastic cells, TNF-α exerts growth inhibitory activities that can be either cytostatic or cytotoxic. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Mouse Tumor Necrosis Factor-α (TNF-α) is derived from the TN3-19.12 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from male Armenian hamster immunized with purified mouse recombinant TNF-α. Tumor necrosis factor (TNF) protein family contains two members: TNF-α (also known as cachectin) and TNF-β (also known as lymphotoxin). These proteins are small molecules with a molecular weight of 17 and 25 kDa respectively and share 30% amino acid homology. TNF-α and -β are secreted from many different cells such as lymphocytes, neutrophils, and macrophages. |
| Immunogen: | purified recombinant mouse TNF-α. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Supplied as a 0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4. |
| Specificity: | Mouse monoclonal clone TN3-19.12 anti-Tumor Necrosis Factor-α antibody recognizes mouse and rat TNF-α (approx. 17 kDa in its monomer form and 51 kDa in its homotrimer form) and cross-reacts with mouse TNF-β. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |