3,3′,5,5′-Tetramethylbenzidine

SIGMA/T2885 - ≥98% (TLC)

Synonym: BM blue; Sure Blue TMB; TMB; TMB Blotting Plus; TMB substrate

CAS Number: 54827-17-7
Empirical Formula (Hill Notation): C₁₆H₂₀N₂
Molecular Weight: 240.34
EC Number: 259-364-6
MDL Number: MFCD00007748
Linear Formula: [-C₆H₂(CH₃)₂-4-NH₂]₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-T2885-100MG	100 mg	$80.30 1/EA	Add To Favorites
45-T2885-1G	1 g	$95.50 1/EA	Add To Favorites
45-T2885-5G	5 g	$332.00 1/EA	Add To Favorites
45-T2885-100G	100 g	$4940.00 1/EA	Add To Favorites

Sigma offers specially formulated TMB liquid substrate systems for ELISA requiring a soluble end-product and for western blotting procedures that require an insoluble end-product. TMB Liquid Substrate System for peroxidase-based ELISA procedures where the soluble blue end product can be read at 370 nm or 655 nm. The addition of an acidic stopping solution produces a yellow color that can be read at 450 nm. The Sigma TMB Substrate System for Membranes produces an insoluble, dark blue end-product, and is not recommended for ELISA or immunohistochemical applications.

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T2885-100G

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98% (TLC)
form	powder
InChI	1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key	UAIUNKRWKOVEES-UHFFFAOYSA-N
mp	168-171 °C (lit.)
Quality Level	200
SMILES string	Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
solubility	ethyl acetate: 50 mg/mL, clear to very slightly hazy
storage temp.	2-8°C

Application:	3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
Application:	3,3′,5,5′-Tetramethylbenzidine has been used: • as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stains the lactobacilli colonies blue in the presence of O2 • as a substrate solution for ELISA (enzyme-linked immunosorbent assay) • in myeloperoxidase activity assay to quantify neutrophil
Biochem/physiol Actions:	3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.
Packaging:	1, 5, 50, 100 g in glass bottle
Packaging:	100 mg in glass bottle
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (TLC)
mp	168-171 °C (lit.)
Storage Temp.	2-8°C
UNSPSC	12352204

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