Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse
SIGMA/T2928 - clone TH-16, ascites fluid
Synonym: Anti-DYT14; Anti-DYT5b; Anti-TYH
MDL Number: MFCD00213051
Product Type: Chemical
Immunocytochemistry JOURNAL CITATION: Acute oral administration of low doses of methylphenidate targets calretinin neurons in the rat septal area. By: García-Avilés, Á., Albert-Gascó, H., et al. in Front Neuroanat, 2015. PubMed ID: 25852493 Image collected and cropped by CiteAb from the following publication, (http://www.frontiersin.org/Neuroanatomy/10.3389/fnana.2015.00033/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry JOURNAL CITATION: Acute oral administration of low doses of methylphenidate targets calretinin neurons in the rat septal area. By: García-Avilés, Á., Albert-Gascó, H., et al. in Front Neuroanat, 2015. PubMed ID: 25852493 Image collected and cropped by CiteAb from the following publication, (http://www.frontiersin.org/Neuroanatomy/10.3389/fnana.2015.00033/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry JOURNAL CITATION: Acute oral administration of low doses of methylphenidate targets calretinin neurons in the rat septal area. By: García-Avilés, Á., Albert-Gascó, H., et al. in Front Neuroanat, 2015. PubMed ID: 25852493 Image collected and cropped by CiteAb from the following publication, (http://www.frontiersin.org/Neuroanatomy/10.3389/fnana.2015.00033/abstract), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:8,000 Monoclonal Anti-Tyrosine Hydroxylase antibody Clone: TH-16 (Cat. No. T2928). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Neuro-2a 2. NT2/D1 3. SH-SY-5Y 4. SK-N-SH 5. PC12
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TH-16, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, sheep, human, monkey, bovine, rabbit, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable using native or SDS-denatured TH. | |
| microarray: suitable | |
| western blot: 1:8,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells. | |
| UniProt accession no. | P07101  |
| Application: | Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in • western blotting • immunofluorescence labelling • immunohistochemical staining • enzyme linked immunosorbent assay (ELISA) • dot blot • immunoprecipitation • immunocytochemistry |
| Application: | Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in immunohistochemistry and double immunofluorescence labeling. |
| Biochem/physiol Actions: | The antibody recognizes an epitope present in the N-terminal region (between aa 40-152) of both rodent and human tryosine hydroxylase. TH-16 detects both the intact subunits and the 59/57 kDa doublet and an array of decreasing molecular weight TH forms resulting from severe proteolysis. |
| Biochem/physiol Actions: | Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson’s disease (PD). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the TH-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Tyrosine hydroxylase (TH) exists in four isoforms in humans. They are are TH-1 (M.W. 55,600), TH-2 (4 additional amino acids; M.W. 56,000), TH-3, (27 additional amino acids; M.W. 58,100), and TH-4 (4 plus 27 additional amino acids; 58,500). Only humans express all four isoforms; all other anthropoids express only the TH-1 and TH-2 isoforms. |
| General description: | Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor. |
| Immunogen: | rat tyrosine hydroxylase (TH). |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |