Anti-mTOR antibody produced in rabbit
SIGMA/T2949 - 1.0-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-FKBP-12 rapamycin associated protein; Anti-FRAP; Anti-RAFT; Anti-SEP
MDL Number: MFCD02263996
Product Type: Chemical
Immunoprecipitation
mTOR was immunoprecipitated from a whole extract of human HEK 293T cells using Anti-mTOR (Cat. No. T2949). The IP samples were separated on SDS-PAGE and blotted with a control anti-rat mTOR. The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and chemiluminescent substrate.
Lane 1: 1.1 μg of Anti-mTOR immunoprecipitates mTOR from a RIPA extract of 300 μg of 293T cells.
Lane 2: negative control (pre-immune serum was used instead of the Anti-mTOR antibody)
mTOR was immunoprecipitated from a whole extract of human HEK 293T cells using Anti-mTOR (Cat. No. T2949). The IP samples were separated on SDS-PAGE and blotted with a control anti-rat mTOR. The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and chemiluminescent substrate.
Lane 1: 1.1 μg of Anti-mTOR immunoprecipitates mTOR from a RIPA extract of 300 μg of 293T cells.
Lane 2: negative control (pre-immune serum was used instead of the Anti-mTOR antibody)
Immunoblotting
Whole cell extract of rat brain was separated on SDS-PAGE and blotted with Anti-mTOR (Cat. No. T2949) at a dilution of 1:4,000. The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and chemiluminescent substrate.
Whole cell extract of rat brain was separated on SDS-PAGE and blotted with Anti-mTOR (Cat. No. T2949) at a dilution of 1:4,000. The antibody was developed with Anti-Rabbit IgG, Peroxidase (Cat. No. A0545) and chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.0-1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 289 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1.0-1.5 μg using 300 μg RIPA extract of human transformed kidney HEK 293T cells |
| microarray: suitable | |
| western blot: 1:2,000 using whole extracts of rat brain tissue | |
| western blot: 1:500 using mouse brain tissue |
| Application: | Anti-mTOR antibody is suitable for use in Western blot (1:1,000 using myotube cell lysates of rainbow trout cultures). The antibody can also be used in indirect immunofluorescence, immunoprecipitation, and microarray. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Anti-mTOR (FRAP) is produced using a peptide corresponding to amino acids 2433-2450 of human mTOR. The corresponding sequence is identical in mouse and differs by one amino acid in rat. The antibody specifically recognizes human, mouse and rat mTOR (289 kDa) and is suitable for immunoblotting, immunoprecipitation and immunofluorescence. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | mTOR is an evolutionary conserved serine/threonine kinase that belongs to the phosphoinositol kinase-related kinase (PIKK) family. This kinase regulates cell growth by initiation of translation in response to nutrients such as amino acids (primarily leucine), growth factors, mitogens and insulin. Translational initiation is mediated by mTOR through S6K1 activation and 4E-BP1 inhibition. mTOR may also modulate several cellular functions such as actin organization, secretion, membrane trafficking, protein degradation, protein kinase C signaling, tRNA synthesis and ribosome biogenesis. Anti-mTOR antibody is specific for human, mouse and rat mTOR (289 kDa). |
| Immunogen: | synthetic peptide corresponding to amino acid residues 2433-2450 of human mTOR. |
| Immunogen: | The corresponding sequence is identical in mouse and differs by one amino acid in rat. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |