Trizma® hydrochloride solution
SIGMA/T3038 - 1 M, BioReagent, Molecular Biology
Synonym: Tris hydrochloride solution
MDL Number: MFCD00012590
Product Type: Chemical
| application(s) | agriculture |
| concentration | 1 M |
| form | solution |
| grade | Molecular Biology |
| impurities | DNase, RNase, NICKase and protease, none detected |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
| InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
| pH | 8.0 |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | Cl.NC(CO)(CO)CO |
| sterility | 0.2 μm filtered |
| Application: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Application: | Trizma® hydrochloride solution may be used in the following studies: • As buffer for the 2-D electrophoresis of rat fibroblast cell. • As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length). • Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp. |
| General description: | Trizma® hydrochloride solution is a useful biological buffer. |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12161700 |