Trizma® hydrochloride solution
SIGMA/T3038 - 1 M, BioReagent, for molecular biology
Synonym: Tris hydrochloride solution
MDL Number: MFCD00012590
Product Type: Chemical
application(s) | agriculture |
concentration | 1 M |
form | solution |
grade | for molecular biology |
impurities | DNase, RNase, NICKase and protease, none detected |
InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
pH | 8.0 |
product line | BioReagent |
Quality Level | 200 ![]() |
SMILES string | Cl.NC(CO)(CO)CO |
sterility | 0.2 μm filtered |
Application: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
Application: | Trizma® hydrochloride solution may be used in the following studies: • As buffer for the 2-D electrophoresis of rat fibroblast cell. • As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length). • Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp. |
General description: | Trizma® hydrochloride solution is a useful biological buffer. |
Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
Other Notes: | Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
UNSPSC | 12161700 |