Tetanus toxin from Clostridium tetani
SIGMA/T3194
CAS Number: 676570-37-9
MDL Number: MFCD01095768
Product Type: Chemical
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| mol wt | 150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond) |
| Quality Level | 100  |
| solubility | H2O: soluble |
| storage temp. | 2-8°C |
| Analysis Note: | Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain). |
| Application: | Tetanus toxin from Clostridium tetani has been used as the exocytosis inhibitor/Golgi-dependent secretion to examine whether secretory carrier membrane protein (SCAMP5) stimulated α-synuclein secretion via the conventional Golgi-dependent pathway. It has also been used in peripheral blood mononuclear cells (PBMCs) culture for T cell proliferation screening. |
| Application: | Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells1. |
| Biochem/physiol Actions: | Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo. |
| Biochem/physiol Actions: | Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals; blocks the release of glycine from inhibitory interneurons of the spinal cord; cleaves synaptobrevin (VAMP-2); blocks synaptic vesicle exocytosis in vivo. |
| Biochem/physiol Actions: | Tetanus toxin can cause spastic paralysis. |
| General description: | Tetanus toxin is also known as tetanospasmin. It is a 150-kDa protein, produced by Clostridium tetani. This protein consists of a light and a heavy chain. |
| Preparation Note: | Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose. |
| Preparation Note: | Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al. |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 + H330 - H311 |
| Precautionary statements | P280 - P301 + P310 + P330 - P302 + P352 + P312 - P304 + P340 + P310 |
| Hazard Codes | T+ |
| Risk Statements | 26/27/28 |
| Safety Statements | 28-36/37-45 |
| RIDADR | UN3462 - class 6.1 - PG 1 - Toxins, extracted from living source |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |