Anti-γ-Tubulin (DQ-19) antibody produced in rabbit
SIGMA/T3195 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-CDCBM4; Anti-GCP-1; Anti-TUBG; Anti-TUBGCP1
MDL Number: MFCD00677366
Product Type: Chemical
Immunofluorescence Centrosomal localization of γ-tubulin in NIH3T3 cells. Fixed cells were stained with Anti-γ-Tubulin (DQ-19) (Cat. No. T3195), (dilution 1:500), followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (Cat. No. 18772) (×100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunofluorescence Centrosomal localization of γ-tubulin in HeLa cells. Fixed cells were stained with Anti-γ-Tubulin (DQ-19) (Cat. No. T3195), (dilution 1:500), followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (Cat. No. 18772) (×100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunoblotting Cell lysates (lanes 1-9) were separated on SDS-PAGE, blotted with Anti-γ-Tubulin (DQ-19) (Cat. No. T3195) (dilution: 1:1,000), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. A431; 2. HeLa; 3. Jurkat; 4. C2C12; 5. Rat2; 6. MDCK; 7. U87; 8. NIH3T3; 9. CHO
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 48 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, human |
| storage temp. | −20°C |
| technique(s) | indirect immunofluorescence: 1:500 using methanol-acetone fixed, cultured chicken fibroblasts. |
| western blot: 1:1,000 using whole cell extract of human epidermal carcinoma A431 cell line. | |
| UniProt accession no. | P23258  |
| Application: | Anti-γ-Tubulin (DQ-19) antibody produced in rabbit has been used in: • western blotting • immunocytochemistry • immunofluorescence |
| Biochem/physiol Actions: | The proper organization of microtubules is essential for cell division and chromosome segregation, directed cell movement, interphase cytoplasmic organization, and other cytoskeletal functions. γ-Tubulin has an essential role in microtubule nucleation by the centrosomes. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-TuRC (γ-tubulin ring complex) binds the microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells. It is a member of the tubulin superfamily of proteins which includes α- and β- tubulin and the newly discovered centrosomal-associated proteins, δ- and ε- tubulin. γ-Tubulin is a part of large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule. Its abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares 28-32% identity with α-tubulin from various organisms, 32-36% identity with β-tubulins and 29-30% identity with δ- and ε tubulins. Some regions including those thought to be involved in GTP) binding are highly conserved among α-, β-, γ-, δ-, and ε-tubulins. |
| Immunogen: | synthetic peptide corresponding to the C-terminus of human γ-tubulin (amino acids 433-451 with N-terminally added lysine), conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |