Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in mouse
SIGMA/T3198 - clone 45418.111, purified immunoglobulin, lyophilized powder
Synonym: Anti-TNF-α
MDL Number: MFCD00162117
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 45418.111, monoclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| isotype | IgG1 |
| Quality Level | 100  |
| species reactivity | rat |
| storage temp. | −20°C |
| technique(s) | capture ELISA: suitable |
| neutralization: suitable | |
| western blot: 1-2 μg/mL | |
| UniProt accession no. | P16599  |
| Application: | Monoclonal Anti-Tumor necrosis factor-α antibody produced in mouse has been used in immunoneutralization study and western blot analysis. |
| Biochem/physiol Actions: | Tumor Necrosis Factor-alpha (TNF-α) is a cytokine produced by macrophages in response to stimulus by LPS. It plays a major role in mediating inflammation, tissue injury, pathogenic shock, innate immunity, apoptosis and autoimmunity. The physiological effects of TNF-α are mediated by receptors of Tumor necrosis factor receptor (TNFR) super family. TNF/TNFR also results in the activation of downstream pathways involving mitogen-activated protein kinase (MAPK), p38(a class of MAPK), c-Jun N-terminal kinase (JNK) and NF-κB (Nuclear Factor kappa-light-chain-enhance Tumor necrosis factor-α (TNFα) affects various cellular processes such as blood-brain barrier, inflammatory, thrombogenic and vascular changes following brain damage. TNFα expression is upregulated in various central nervous system disorders, including Alzheimer’s disease, multiple sclerosis, Parkinson’s disease, meningococcal meningitis and human immunodeficiency virus (HIV) infection. Elevated expression of TNFα in the brain and blood in response to lipopolysaccharide might increase brain stem thrombosis, hemorrhage and stroke sensitivity/risk in hypertensive rats. Tumor necrosis factor-α activates polymorphonuclear leukocyte NADPH oxidase, thereby facilitates development of systemic oxidative stress (OS), inflammation and hypertension in rats. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The antibody has the ability to neutralize the biological activity of rat TNF-α.and may also be used as a capture antibody in ELISAs. |
| Immunogen: | purified recombinant rat TNF-α expressed in E. coli. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |