Trizma® hydrochloride
SIGMA/T3253 - reagent grade, ≥99.0% (titration), crystalline
Synonym: TRIS HCl; TRIS hydrochloride; Tris(hydroxymethyl)
CAS Number: 1185-53-1
Empirical Formula (Hill Notation): C4H11NO3 · HCl
Molecular Weight: 157.60
EC Number: 214-684-5
MDL Number: MFCD00012590
Linear Formula: NH2C(CH2OH)3 · HCl
Product Type: Chemical
FT-IR Spectra for TRIS HCl.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T3253-100G
This image depicts SKU: T3253-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T3253-250G
This image depicts SKU: T3253-250G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T3253-500G
This image depicts SKU: T3253-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T3253-1KG
This image depicts SKU: T3253-1KG
| absorption | ≤0.05 at 290 nm at 40% |
| application(s) | diagnostic assay manufacturing life science and biopharma sample preparation |
| assay | ≥99.0% (titration) |
| color | white |
| density | 1.39 g/cm3 at 20—25 °C |
| 1.41 g/cm3 at 20—25 °C | |
| form | crystalline |
| grade | reagent grade |
| impurities | ≤0.5% water (Karl Fischer) |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
| InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
| mp | 150-152 °C |
| pKa (25 °C) | 8.1 |
| Quality Level | 400  |
| SMILES string | Cl.NC(CO)(CO)CO |
| solubility | H2O: soluble, clear, colorless |
| storage condition | dry at room temperature |
| suitability | suitable for mass spectrometry |
| technique(s) | affinity chromatography: suitable |
| useful pH range | 7.0-9.0 |
| Application: | Trizma® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure. |
| Features and Benefits: | • Ideal as a Biological buffer for Cell Biology and Biochemical research • Effective Buffering from pH 7-9 with a pKa of 8.1 (25 °C) • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications |
| General description: | Tris HCl, or Trizma® hydrochloride, is a widely utilized acidic buffer in the physiological pH range of 7.3 to 7.5. With a pKa of 8.08 at 25°C, it offers a pH range of 7.0 to 9.0, aligning with living organisms′ typical pH. Essential in various biological, cell biology, and biochemistry applications, Tris HCl is crucial for effective pH control in buffer solutions. Notably, Tris base and Tris hydrochloride alone lack sufficient buffering capacity, prompting their combination within the pH range of 7 to 9. Tris buffers play a key role in techniques like protein electrophoresis and western blotting, serving in low ionic strength buffers for protein transfer. They are also integral to SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, especially in TAE and TBE buffers, are extensively employed in DNA agarose electrophoresis. These buffers are pivotal in diverse applications, including biochemistry, protein purification, and electrophoresis, showcasing the versatility and importance of Tris HCl in maintaining optimal pH for biochemical and cell biology research. |
| Legal Information: | DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Tyvek is a registered trademark of E. I. du Pont de Nemours and Company |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Other Notes: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 100, 250, 500 g in poly bottle |
| Packaging: | 5, 10, 25, 50 kg in poly drum |
| Packaging: | The DRYPOUR®packaging system for high-quality hygroscopic salts DRYPOUR®packaging: • The current DRYPOUR®packaging configuration is in a 50L nestable drum • 25KG-DP are the standard prepack offering • Smaller volumes can be put in the same drum for variable configurations The benefits of DRYPOUR®: • Improves operational efficiency by reducing material preparation time and increasing operator safety • Minimizes caking of hygroscopic salts • Offers triple protection: against moisture inside and outside, and against contamination How does the DRYPOUR®packaging system Improve your operational efficiency? • This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation • PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek®liner • Desiccant bags are firmly integrated into the polyethylene liner • Triple protection delivers two invaluable results:
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| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% (titration) |
| mp | 150-152 °C |
| Density | 1.39 g/cm3 at 20—25 °C; 1.41 g/cm3 at 20—25 °C |
| UNSPSC | 12161700 |