Trizma® hydrochloride
SIGMA/T3253 - reagent grade, ≥99.0% (titration), crystalline
Synonym: TRIS HCl; TRIS hydrochloride; Tris(hydroxymethyl)
CAS Number: 1185-53-1
Empirical Formula (Hill Notation): C4H11NO3 · HCl
Molecular Weight: 157.60
EC Number: 214-684-5
MDL Number: MFCD00012590
Linear Formula: NH2C(CH2OH)3 · HCl
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T3253-100G
This image depicts SKU: T3253-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T3253-250G
This image depicts SKU: T3253-250G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T3253-500G
This image depicts SKU: T3253-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T3253-1KG
This image depicts SKU: T3253-1KG
| absorption | ≤0.05 at 290 nm at 40% |
| application(s) | diagnostic assay manufacturing |
| assay | ≥99.0% (titration) |
| form | crystalline |
| grade | reagent grade |
| impurities | ≤0.5% water (Karl Fischer) |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
| InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
| mp | 150-152 °C |
| pKa (25 °C) | 8.1 |
| Quality Level | 400  |
| SMILES string | Cl.NC(CO)(CO)CO |
| solubility | H2O: soluble, clear, colorless |
| useful pH range | 7.0-9.0 |
| Application: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Application: | Trizma® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure. |
| General description: | Trizma® hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution. |
| Legal Information: | Trizma is a registered trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Easily compare specifications for Trizma HCl products with the Trizma HCl specification table . |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 100, 250, 500 g in poly bottle |
| Packaging: | 5, 10, 25, 50 kg in poly drum |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% (titration) |
| mp | 150-152 °C |
| UNSPSC | 12161700 |