Monoclonal Anti-Talin antibody produced in mouse
SIGMA/T3287 - clone 8d4, ascites fluid
Synonym: Monoclonal Anti-Talin
MDL Number: MFCD00164744
Product Type: Chemical
Western Blotting JOURNAL CITATION: αV-integrins are required for mechanotransduction in MDCK epithelial cells. By: Teräväinen, T. P., Myllymäki, S. M., et al. in PLoS One, 2013. PubMed ID: 23977051 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0071485), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Immunofluorescent staining of rat embryo fibroblasts using Monoclonal Anti-Talin, (clone no. 8d4, Cat. No. T3287), showing reactivity with focal adhesions. From Dr. C. Otey, University of North Carolina, Chapel Hill, NC.
Western Blotting Chicken gizzard extract were separated on SDS-PAGE and probed with Monoclonal Anti-Talin antibody produced in Mouse, Clone: 8d4 (Cat. No. T3287). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:100 antibody 2. 1:200 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 8d4, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 225-235 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, frog, rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: 1:500 using cultured Rat2 cells | |
| western blot: 1:100 using chicken gizzard extract | |
| UniProt accession no. | Q9Y490  |
| Application: | Monoclonal Anti-Talin may be used for the localization of talin using various immunochemical assays such as immunoblot, immunocytochemistry, immunofluorescence, enzyme linked immunosorbent assay (ELISA) and for microinjection and transfection studies. The antibody recognizes native talin and can be used for immunoprecipitation. |
| Biochem/physiol Actions: | Talin 1 (TLN1) is a cytoskeletal protein, which plays a vital role in cancer cell adhesion, migration and invasion. It is also involved in integrin activation and it facilitates the adhesion of integrin to actin cytoskeleton. TLN1 might act as a potential prognostic biomarker in nasopharyngeal carcinoma (NPC). Overexpression of TLN1 is observed in endometrioid carcinoma, oral squamous cell carcinoma (OSCC), prostate cancer and hepatocellular carcinoma. Serum TLN1 functions as a potential biomarker of hepatocellular carcinoma (HCC). Talin 1 (TLN1) is highly expressed in glioblastoma multiforme (GBM). The Inhibition of TLN1 activity helps in diminishing malignant features of GBM cells. It also plays an important role in regulation of, Bevacizumab (anti-angiogenic agent)-resistance. |
| Biochem/physiol Actions: | Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Talin (mouse IgG1 isotype) is derived from the 8d4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Talin is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. |
| General description: | Talin 1 (TLN1) gene mapped to human chromosome 9p13.3, codes for a 270kDa cytoskeletal protein containing 2541 amino acids. Talin-1 is widely expressed in the kidney, liver, spleen, stomach, lung and vascular smooth muscle. The TLN1 antiparallel dimeric structure is characterized with a globular head containing four-point-one, ezrin, radixin, moesin (FERM) domain and rod domain involved in dimer formation. |
| Immunogen: | purified chicken gizzard talin. |
| Specificity: | Reacts specifically with mammalian and avian talin. In immunoblotting, the antibody recognizes an epitope located on the 225 kDa chicken talin molecule and the 190 kDa fragment obtained by protease cleavage. It also localizes the slightly higher molecular weight talin in human platelets. The antibody labels focal adhesions, membrane ruffles, and ventral streaks in methanol/acetone fixed cultured fibroblasts. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |