Anti-γ-Tubulin (AK-15) antibody produced in rabbit
SIGMA/T3320 - IgG fraction of antiserum, buffered aqueous solution
MDL Number: MFCD00677366
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunofluoresence. HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Anti-γ-Tubulin (AK-15) antibody produced in Rabbit (Cat. No. T3320) (A). The antibody developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody, and 1 μg/mL Monoclonal Anti-γ-Tubulin antibody produced in Mouse, Clone: GTU-88 (Cat. No. T5326) (B). The antibody was developed using Goat Anti-Mouse IgG, Cy3 Conjugate antibody. Results Two Anti-γ-Tubulin antibodies, T3320 (A) and T5326 (B) target different regions of γ-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence
Rat1 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Rabbit Anti-γ-Tubulin (AK-15) (Cat. No. T3320). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate.
Rat1 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Rabbit Anti-γ-Tubulin (AK-15) (Cat. No. T3320). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate.
Immunofluorescence
HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Rabbit Anti-γ-Tubulin (AK-15) (Cat. No. T3320). The antibody was developed using Goat Anti-Rabbit IgG, Atto-488 conjugate (Cat. No. 18772).
HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Rabbit Anti-γ-Tubulin (AK-15) (Cat. No. T3320). The antibody was developed using Goat Anti-Rabbit IgG, Atto-488 conjugate (Cat. No. 18772).
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:1,000 Rabbit Anti-γ-Tubulin (AK-15) (Cat. No. T3320). The antibody was developed using Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa; 2. NIH-3T3;3. MDCK; 4. CHO; 5. Rat2 ; 6. A431; 7. JURKAT; 8. U87; 9. C2C12
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 48 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, frog, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 1:1,000 using methanol-acetone fixed, cultured rat, RAT-2 cells |
| microarray: suitable | |
| western blot: 1:1,000 using whole cell extract of the human epitheloid carcinoma HeLa cell line. | |
| western blot: 1:5,000 using Xenopus eggs extract | |
| UniProt accession no. | P23258  |
| Application: | Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in following studies: • Western blot. • Immunofluorescence. |
| Application: | Anti-γ-Tubulin (AK-15) antibody produced in rabbit has been used in: • western blotting • immunocytochemistry (immunofluorescence staining • immunofluorescence |
| Biochem/physiol Actions: | γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression ofγ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | γ-Tubulin (48 kDa) is a highly conserved protein. The microtubule cytoskeleton consists of a dynamic, highly polarized network of microtubules filaments, microtubule-associated proteins, microtubule motors, and microtubule-organizing proteins. γ-Tubulin is found as part of a large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule. |
| General description: | γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ -Tubulin consists of two isoforms tubulinγ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ -tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |