Thermitase from Bacillus cereus
SIGMA/T3330 - recombinant, expressed in Bacillus subtilis, enzyme ≥5.0 units/mg protein
Product Type: Chemical
| enzyme activity | ≥5.0 units/mg protein |
| foreign activity | DNase, RNase, none detected |
| form | solid |
| mol wt | 33 kDa |
| recombinant | expressed in Bacillus subtilis |
| storage temp. | −20°C |
| Application: | Thermitase is used for the isolation of RNA and DNA (plasmid and genomic). Thermitase has been used in a study to assess the development and application of 15N-tracer substances for measuring the whole-body protein turnover rates. Thermitase has also been used in a study to investigate a computational procedure for transferring a binding site onto an existing protein scaffold. |
| Biochem/physiol Actions: | Thermitase is a thermostable (4-60°C) serine protease that remains active over a wide pH range of 4-12.5. This protease retains its activity in the presence of EDTA, SDS, or Triton™ X-100. |
| General description: | Thermitase from Bacillus cereus is a protease that inactivates Rnases, Dnases and enzymes in reactions. |
| Legal Information: | Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow |
| Physical form: | Supplied as a solution in 20mM Tris-HCl (pH 7.4), 1mM CaCl2 and 50% glycerol. |
| Unit Definition: | One unit is defined as the amount of enzyme required to solubilize 1 absorbance unit (595nm) of milk in 60 minutes at 37°C in a total reaction volume of 200ul. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | enzyme activity: ≥5.0 units/mg protein |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |