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3,3′,5,5′-Tetramethylbenzidine dihydrochloride

SIGMA/T3405 - tablet, 1 mg substrate per tablet

Synonym: 4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

CAS Number: 64285-73-0
Empirical Formula (Hill Notation): C16H20N2 · 2HCl
Molecular Weight: 313.27
EC Number: 264-769-6
MDL Number: MFCD00012961
Linear Formula: C16H20N2 · 2HCl
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-T3405-5TAB 5 tabs
 $70.80
1/EA		 Add To Favorites
45-T3405-50TAB 50 tabs
 $234.00
1/EA		 Add To Favorites
45-T3405-100TAB 100 tabs
 $403.00
1/EA		 Add To Favorites

 

form tablet
InChI 1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
InChI key NYNRGZULARUZCC-UHFFFAOYSA-N
mp ≥300 °C
Quality Level 200 
SMILES string Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
storage temp. 2-8°C
Application: 3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
Application: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
• anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
• anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
• in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
mp ≥300 °C
Storage Temp. 2-8°C
UNSPSC 12352204

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