3,3′,5,5′-Tetramethylbenzidine dihydrochloride

SIGMA/T3405 - tablet, 1 mg substrate per tablet

Synonym: 4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

CAS Number: 64285-73-0
Empirical Formula (Hill Notation): C₁₆H₂₀N₂ · 2HCl
Molecular Weight: 313.27
EC Number: 264-769-6
MDL Number: MFCD00012961
Linear Formula: C₁₆H₂₀N₂ · 2HCl
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-T3405-5TAB	5 tablets	$64.20 1/EA	Add To Favorites
45-T3405-50TAB	50 tablets	$211.00 1/EA	Add To Favorites
45-T3405-100TAB	100 tablets	$365.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

form	tablet
InChI	1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
InChI key	NYNRGZULARUZCC-UHFFFAOYSA-N
mp	≥300 °C
Quality Level	200
SMILES string	Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
storage temp.	2-8°C

Application:

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm.

Application:

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
• anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
• anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
• in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

mp	≥300 °C
Storage Temp.	2-8°C
UNSPSC	12352204

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