Tumor Necrosis Factor Soluble Receptor I (TNF sRI)/Fc Chimera human
SIGMA/T3439 - >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
MDL Number: MFCD02689207
Product Type: Chemical
TNF alpha Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
Apoptosis Pathway Research areas: Apoptosis, Inflammation and Infection, Cancer biology, Immunology, Developmental biology Disease areas: Autoimmune disorders, cancer, cardiomyopathy, HIV, hepatitis, tuberculosis, ulcerative colitis See also: T6674; T0157; T7539; 11371843001; SRP8024; 11088939001
| assay | >95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | 48 kDa |
| packaging | pkg of 50 μg |
| potency | 0.4-1 ng/mL ED50 |
| Quality Level | 100  |
| recombinant | expressed in NSO cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P20333  |
| Analysis Note: | The biological activity is measured by its ability to neutralize apoptosis of mouse L-929 cells treated with 0.25 ng/ml recombinant human TNF-α. |
| Application: | Tumor Necrosis Factor Soluble Receptor I (TNF sRI)/Fc Chimera human may be used to study signaling of apoptosis and NF-κB activation. |
| Biochem/physiol Actions: | Tumor Necrosis Factor Soluble Receptor I (TNF sRI)/Fc Chimera human belongs to TNF family of receptors and has a pivotal role in neutralizing the biological activities of both TNF-α and TNF-β. Soluble tumor necrosis factor receptor along with intrathecal interleukin-1 receptor antagonist shows an anti-allodynic action in rat L5 spinal nerve transection model of neuropathic pain. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 2.5 mg bovine serum albumin. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |