Thiolutin
SIGMA/T3450 - from Streptomyces luteosporeus, ≥95% (HPLC)
Synonym: Farcinicin; N-
CAS Number: 87-11-6
Empirical Formula (Hill Notation): C8H8N2O2S2
Molecular Weight: 228.29
MDL Number: MFCD07370147
Linear Formula: C8H8N2O2S2
Product Type: Chemical
antibiotic activity spectrum | fungi |
assay | ≥95% (HPLC) |
biological source | Streptomyces luteosporeus |
form | solid |
InChI | 1S/C9H10N2O2S2/c1-3-6(12) |
InChI key | UGZYFXMSMFMTSM-UHFFFAOYSA |
mode of action | enzyme | inhibits |
Quality Level | 200 ![]() |
shipped in | wet ice |
SMILES string | CCC(=O)NC1=C2SSC=C2N(C)C1 |
solubility | DMSO: 0.90 - 1.10 mg/ml, clear, yellow |
storage temp. | −20°C |
Application: | Thiolutin has been used as a polymerase II inhibitor: • to study its effects on yeast cells to calculate transcript half-life • to study its effects on transcription during germination in budding yeast • to study its effects on cell adhesion in zebrafish |
Biochem/physiol Actions: | Sulfur-containing antibiotic, which is a potent inhibitor of bacterial and yeast RNA polymerases. It was found to inhibit in vitro RNA synthesis directed by all three yeast RNA polymerases (I, II, and III). Thiolutin is also an inhibitor of mannan and glucan formation in Saccharomyces cerevisiae and used for the analysis of mRNA stability. Studies have shown that thiolutin inhibits adhesion of human umbilical vein endothelial cells (HUVECs) to vitronectin and thus suppresses tumor cell-induced angiogenesis in vivo. |
Packaging: | 1 mg in glass bottle |
Preparation Note: | Thiolutin dissolves in DMSO at 0.90 - 1.10 mg/ml to yield a clear, yellow solution. |
Symbol | ![]() |
Signal word | Danger |
Hazard statements | H300 |
Precautionary statements | P264 - P301 + P310 |
Hazard Codes | T+ |
Risk Statements | 28 |
Safety Statements | 45 |
RIDADR | UN 2811 6.1 / PGII |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (HPLC) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |