Anti-Tubulin antibody produced in rabbit
SIGMA/T3526 - whole antiserum
Synonym: Anti-K-ALPHA-1
MDL Number: MFCD00145921
Product Type: Chemical
Immunofluorescence
Double labeling of cultured chicken fibroblasts for vinculin using Monoclonal Anti-Human Vinculin (Clone no. hVIN-1, Cat. No. V9131) and Rabbit Anti-Tubulin (Cat. No. T3526) (green).
From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg, Germany.
Double labeling of cultured chicken fibroblasts for vinculin using Monoclonal Anti-Human Vinculin (Clone no. hVIN-1, Cat. No. V9131) and Rabbit Anti-Tubulin (Cat. No. T3526) (green).
From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg, Germany.
Western Blotting
Chicken gizzard extract was separated on SDS-PAGE and probed with Rabbit Anti-Tubulin (Cat. No. T3526). The antibody was developed using Goat Anti-Rabit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 1:500 antibody
2. Negative control: without first Antibody
Chicken gizzard extract was separated on SDS-PAGE and probed with Rabbit Anti-Tubulin (Cat. No. T3526). The antibody was developed using Goat Anti-Rabit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 1:500 antibody
2. Negative control: without first Antibody
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, mouse |
| storage temp. | −20°C |
| technique(s) | indirect immunofluorescence: 1:80 using cultured fibroblasts |
| western blot: 1:200 using chicken gizzard extract or mouse brain extract | |
| UniProt accession no. | P68363  |
| Application: | Anti-Tubulin antibody produced in rabbit has been used in immunoblotting and indirect immunofluorescence. |
| Biochem/physiol Actions: | αβ-tubulin heterodimers helps in formation of microtubules. Tubulin dimers exhibits GTPase activity, assembly and disassembly properties. It facilitates the construction of the flagellar apparatus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Microtubules are composed of tubulin and several additional proteins designated microtubule associated proteins (MAPs). Tubulin is a heterodimer which consists of an α-chain and a β-chain, both of which have a molecular weight of approximately 55,000. Microtubules form an elaborate cytoplasmic network during interphase and are reorganized into the mitotic spindle during cell division. |
| General description: | Tubulin is an extremely conserved and negatively charged protein. It is expressed in all eukaryotic cells, but mainly in brains. |
| Immunogen: | microtubule proteins from brains of 13 day old chicken embryos |
| Specificity: | The product stains tubulin in cultured chicken fibroblasts or 3T3 mouse fibroblasts. Additional bands may be stained in immunoblotting. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |