Anti-γ-Tubulin antibody produced in rabbit
SIGMA/T3559 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-γ-Tubulin
Product Type: Chemical
Western Blotting JOURNAL CITATION: A mechanical model of cell segregation driven by differential adhesion. By: Taylor, W. R., Morley, R., et al. in PLoS One, 2012. PubMed ID: 22952652 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0043226), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Independent Antibodies Immunofluorescence. CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:5,000 Anti-γ-Tubulin antibody produced in Rabbit (Cat. No. T3559) (A). The antibody developed using Goat Anti-Rabbit IgG, Cy3 conjugate antibody, and 1:5,000 Anti-γ-Tubulin antibody produced in Rabbit (Cat. No. T5192) (B). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate antibody. Results Two Anti-γ-Tubulin antibodies, T3559 (A) and T5192 (B), target different regions of γ-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence Centrosomal localization of γ-tubulin in NIH3T3 cells. Fixed cells were stained with Anti-γ-Tubulin (Cat. No. T3559), (dilution 1:1500), followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (Cat. No. 18772) (×100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunofluorescence Centrosomal localization of γ-tubulin in HeLa cells. Fixed cells were stained with Anti-γ-Tubulin (Cat. No. T3559), (dilution 1:1,500), followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (Cat. No. 18772) (×100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunofluorescence Centrosomal localization of γ-tubulin in Rat2 cells. Fixed cells were stained with Anti-γ-Tubulin (Cat. No. T3559), (dilution 1:1500), followed by Goat Anti-Rabbit IgG, Atto448 dye conjugate (Cat. No. 18772) (×100 magnification). Actin staining with phalloidin rhodamine conjugate. Nuclear staining with DAPI.
Immunoblotting Cell lysates (lanes 1-8) were separated on SDS-PAGE, blotted with Anti-γ-Tubulin (Cat. No.T3559) (dilution: 1:1,000), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. A431; 2. HeLa; 3. Jurkat; 4. C2C12; 5. Rat2; 6. U87; 7. NIH3T3; 8. CHO
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 48 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:5,000 using methanol/acetone-fixed chicken fibroblasts |
| microarray: suitable | |
| western blot: 1:1,000 using cultured A431 whole cell extracts | |
| UniProt accession no. | P23258  |
| Application: | Anti-γ-Tubulin antibody produced in rabbit has been used in • immunofluorescence analysis • western blotting • immunohistochemistry |
| Application: | Anti-γ-Tubulin polyclonal antibody is suitable for immunoblotting of materials such as A431 cultured cells whole extract (g-tubulin, 48 kDa), and immunocytochemical staining of cells such as cultured chicken fibroblast cells. |
| Biochem/physiol Actions: | γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. γ-Tubulin is not a member of microtubule like α- and β-tubulin. It functions as the microtubule nucleator at the microtubule organizing centre (MTOC). It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. |
| Biochem/physiol Actions: | Anti-gamma-Tubulin recognizes an epitope located in the N-terminal region of gamma-tubulin (amino acids 38-53). Staining of gamma-tubulin (48 kDa) by immunoblotting is specifically inhibited with gamma-tubulin peptide (human, amino acids 38-53 with C-terminally added lysine). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. It is related to α- and β-tubulin and is a member of the tubulin superfamily of proteins. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares approximately 28-32% identity with α-tubulin from various organisms and 32-36% identity with β-tubulins. Some regions are highly conserved among α-, β- and γ-tubulins. |
| Immunogen: | synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |