RANK Ligand/TRANCE human
SIGMA/T3573 - >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder
Synonym: Osteoclast Differentiation Factor (ODF); Osteoprotegerin Ligand (OPGL); Receptor Activator of NF-KB Ligand (RANKL); TNF-related activation-induced cytokines (TRANCE)
CAS Number: 207621-35-0
MDL Number: MFCD01867142
Product Type: Chemical
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| application(s) | cell analysis |
| assay | >90% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | calculated mol wt 23 kDa |
| glycolysylated recombinant product ~35 kDa by SDS-PAGE | |
| packaging | pkg of 10 μg |
| potency | 1.5-7.5 ng/mL ED50 |
| Quality Level | 200  |
| recombinant | expressed in NSO cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | O14788  |
| Analysis Note: | The biological activity is measured by its ability to induce osteoclast differentiation on mouse splenocytes. |
| Application: | RANK Ligand/TRANCE human can be used in in vitro osteoclastogenic assays for osteoclast differentiation. |
| Application: | RANK Ligand/TRANCE human has been used to stimulate differentiation. |
| Biochem/physiol Actions: | Member of the TNF superfamily; induces activation of the c-jun N-terminal kinase, enhances T-cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis; cell surface receptor for RANK Ligand. |
| Biochem/physiol Actions: | RANK Ligand (receptor activator of NF-kB ligand (RANKL) also referred to as TNF-related activation induced cytokines (TRANCE) or osteoprotegerin ligand is a protein that belongs to tumor necrosis factor (TNF) family. It stimulates the mature dendritic cells and induces the cytokine production. RANKL also facilitates bone remodelling and possess an angiogenic activity. |
| Biochem/physiol Actions: | RANK Ligand/TRANCE, a member of the TNF superfamily, induces activation of the c-jun N-terminal kinase, enhances T-cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis. Mouse and human RANK Ligand share 85% amino acid identity. RANK is the cell surface receptor for RANK Ligand. |
| General description: | Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) is also known as receptor activator of nuclear factor-κB ligand (RANKL). It is a type II transmembrane signaling receptor. It has a molecular weight of around 35kDa. This protein contains an amino-terminal intracellular tail and a carboxy-terminal extracellular region, that bears a connecting stalk and a receptor-binding domain. Tnfsf11 is located on human chromosome 13q14.11. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 20 mM MOPS and 500 mM NaCl, pH 6.5, with 50 μg BSA per 1 μg as a carrier protein. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |