Tyrosinase from mushroom
SIGMA/T3824 - lyophilized powder, ≥1000 unit/mg solid
Synonym: Catechol Oxidase; Monophenol Monooxygenase; Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase; Polyphenol Oxidase
CAS Number: 9002-10-2
MDL Number: MFCD00082118
Product Type: Chemical
Tyrosinase in a copper-containing monophenol oxygenase. with activity for both catechols and cresol.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T3824-250KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T3824-25KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T3824-1MU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T3824-500KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T3824-50KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T3824-25KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T3824-50KU
| application(s) | diagnostic assay manufacturing |
| biological source | fungus (mushroom) |
| form | lyophilized powder |
| mol wt | 119.5 kDa by electrophoresis |
| 128 kDa by sedimentation velocity diffusion | |
| 133 kDa by LS | |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥1000 unit/mg solid |
| storage temp. | −20°C |
| Application: | Tyrosinase activity has been assessed in a study that developed an alternative therapeutic agent for treating hyperpigmentation. Tyrosinase has also been used in a study to investigate the oculocutaneous albinism phenotype in the Pakistani population. |
| Biochem/physiol Actions: | Converts tyrosine to |
| General description: | Isoelectric point (pI): 4.7-5 pH optimum is 6-7 Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis. Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion. |
| General description: | Tyrosinase is a copper-containing oxidase with activity for catechols and cresol. Tyrosinase participates in the conversion of tyrosine to dihydroxyphenylalanine (DOPA), and polymerization of DOPA leads to melanin formation. Tyrosinase has a role in browning reactions. The enzyme is said to have two binding sites for aromatic substrates and a distinct binding site for oxygen-copper. |
| Packaging: | 25000, 50000 units in glass bottle |
| Packaging: | 250000, 500000, 1000000 units in poly bottle |
| Physical properties: | Isoelectric point (pI): 4.7-5 pH optimum is 6-7 Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis. |
| Unit Definition: | One unit = ΔA280 of 0.001 per min at pH 6.5 at 25 °C in 3 mL reaction mix containing |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1000 unit/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.14.18.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |