Trypsin−Agarose
SIGMA/T4019 - buffered aqueous suspension, from bovine pancreas trypsin
MDL Number: MFCD00082094
Product Type: Chemical
| biological source | bovine pancreas (trypsin) |
| concentration | ≥15 units/mL (packed gel) |
| extent of labeling | ≥15 units per mL (packed gel) |
| form | buffered aqueous suspension |
| matrix | beaded agarose |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA. |
| General description: | The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-su |
| Physical form: | Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative |
| Preparation Note: | TPCK-treated |
| Unit Definition: | One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38-42/43 |
| Safety Statements | 23-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |