Trypsin−Agarose
SIGMA/T4019 - buffered aqueous suspension, from bovine pancreas trypsin
MDL Number: MFCD00082094
Product Type: Chemical
biological source | bovine pancreas (trypsin) |
concentration | ≥15 units/mL (packed gel) |
extent of labeling | ≥15 units per mL (packed gel) |
form | buffered aqueous suspension |
matrix | beaded agarose |
Quality Level | 200 ![]() |
storage temp. | 2-8°C |
Application: | An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA. |
General description: | The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-su |
Physical form: | Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative |
Preparation Note: | TPCK-treated |
Unit Definition: | One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay). |
Symbol | ![]() |
Signal word | Warning |
Hazard statements | H317 |
Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
Hazard Codes | Xi |
Risk Statements | 36/37/38-42/43 |
Safety Statements | 23-36/37-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
UNSPSC | 12352204 |