Monoclonal Anti-β-Tubulin antibody produced in mouse
SIGMA/T4026 - clone TUB 2.1, ascites fluid
Synonym: Monoclonal Anti-β-Tubulin
Product Type: Chemical
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Biochemical, electron microscopic and immunohistological observations of cationic detergent-extracted cells: detection and improved preservation of microextensions and ultramicroextensions. By: Nakamura, F. in BMC Cell Biol, 2001. PubMed ID: 11425343 Image collected and cropped by CiteAb from this publication, provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Independent Antibodies Chicken fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:2,000 Monoclonal Anti-β-Tubulin antibody produced in Mouse, Clone: 2-28-33 (Cat. No. T5293) (A). The antibody was developed using 1:20 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262), and 1:400 Monoclonal Anti-β-Tubulin antibody produced in Mouse, Clone: TUB 2.1 (Cat. No. T4026) (B). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262). Results Two Anti-β-Tubulin antibodies, T5293 (A) and T4026 (B) target different regions of β-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence Colon cancer cells stained with Monoclonal Anti-β-Tubulin, clone no. TUB 2.1 (Cat. No. T4026) and Hoechst 33258 (Cat. No. B2883). From L. Fazikas, Immunogen, Inc., Dept. of Cell Biology, Cambridge, MA.
The reconstructed confocal image of tripolar mitotic rat hepatoma cells (FAO cells) stained with Monoclonal Anti-β-Tubulin, clone no. TUB 2.1 (Cat. No. T4026) shows spindle apparatus having three centers of mitosis (blue color). Associated chromosomes are yellow colored. From M. Cammer, Albert Einstein College of Medicine, Image Analysis Facility, Anatomy & Structural Biology, Bronx, NY.
Immunofluorescence
Confocal microscopy of rat hepatoma cell line (FAO cells) stained with Monoclonal Anti-β-Tubulin, clone No. TUB 2.1 (Cat. No. T4026). Nuclei and chromosomes are stained with propidium iodide (red). Microtubules (green) are seen in cytoplasm of non-dividing cells and in bipolar and tripolar mitotic apparatus of dividing cells.
From P. Novikoff, Albert Einstein College of Medicine, Bronx, NY.
Confocal microscopy of rat hepatoma cell line (FAO cells) stained with Monoclonal Anti-β-Tubulin, clone No. TUB 2.1 (Cat. No. T4026). Nuclei and chromosomes are stained with propidium iodide (red). Microtubules (green) are seen in cytoplasm of non-dividing cells and in bipolar and tripolar mitotic apparatus of dividing cells.
From P. Novikoff, Albert Einstein College of Medicine, Bronx, NY.
Immunofluorescence Human monocytes cultured on hardware MIA in real microgravity on board International Space Station, labeled with Monoclonal Anti-β-Tubulin, clone TUB 2.1 (Cat. No. T4026) (green) and DAP for the DNA. From Giuseppe Pani, Dipartmento di Scienze Fisiologiche, Biochimiche e Cellulari, Sassari University, Sassari, Italy.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:200 Monoclonal Anti-β-Tubulin, Clone: TUB 2.1 (Cat. No. T4026). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. U87; 3. P19; 4. Neuro-2a; 5. PC-12; 6. COS-7; 7. CHO; 8. MDBK; 9. MDCK
Immunoblotting
Whole cell extract of chicken gizzard was separated on SDS-PAGE and blotted with Monoclonal Anti-β-tubulin (Cat. No. T4026). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A9917) and AEC substrate.
Lanes
A: Antibody dilution 1:200
B: Negative control (only secondary antibody)
Whole cell extract of chicken gizzard was separated on SDS-PAGE and blotted with Monoclonal Anti-β-tubulin (Cat. No. T4026). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A9917) and AEC substrate.
Lanes
A: Antibody dilution 1:200
B: Negative control (only secondary antibody)
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | TUB 2.1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| enhanced validation | independent ( Antibodies) Learn more about Antibody Enhanced Validation  |
| isotype | IgG1 |
| mol wt | antigen 55 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster |
| storage temp. | −20°C |
| technique(s) | indirect immunofluorescence: 1:200 using cultured chicken fibroblasts |
| western blot: 1:100 using human or chicken fibroblast cell extract |
| Application: | Monoclonal Anti β-Tubulin antibody produced in mouse has been used in following studies: • Fluorescence-activated cell sorting (FACS) • Immunoblotting • Immunofluorescence Analysis |
| Biochem/physiol Actions: | Mutation in the β-tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β- 1 tubulin also known as Class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-Tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)). Monoclonal Anti-b-Tubulin (mouse IgG1 isotype) is derived from the hybridoma TUB 2.1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified rat brain tubulin. |
| Immunogen: | tubulin from rat brain. |
| Physical form: | Supplied as ascites fluid with 15 mM sodium azide as a preservative. |
| Specificity: | The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |