JC-1
SIGMA/T4069 - solid
Synonym: 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide; 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide; CBIC2(3)
CAS Number: 3520-43-2
Empirical Formula (Hill Notation): C25H27Cl4IN4
Molecular Weight: 652.23
MDL Number: MFCD00188475
Linear Formula: C25H27Cl4IN4
Product Type: Chemical
| ε (extinction coefficient) | ≥175000 at 511-517 nm in methanol |
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥97% |
| form | solid |
| InChI | 1S/C25H27Cl4N4.HI/c1-5-30 |
| InChI key | FYNNIUVBDKICAX-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | [I-].CCN1C(=CC=Cc2n(CC) |
| storage temp. | room temp |
| Application: | A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density. |
| Application: | The cyanine dye JC-1 has been used to determine mitochondrial transmembrane potential. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |