Anti-Rat IgG (whole molecule)−TRITC antibody produced in rabbit
SIGMA/T4280 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00162814
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:100 Rat Anti-Human IgG followed by 1:100 Anti-Rat IgG (whole molecule)-TRITC antibody produced in Rabbit (Cat. No. T4280).
Immunofluorescence Human Periferal Blood Lymphocytes probed and developed using 1:100 Rat Anti-Human IgG followed by 1:200 Anti-Rat IgG (whole molecule)-TRITC antibody produced in Rabbit (Cat. No. T4280).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | TRITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | direct immunofluorescence: 1:100 using human peripheral blood |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tonsils |
| Application: | Anti-Rat IgG (whole molecule)-TRITC antibody has been used in • confocal microscopy • immunohistochemistry • immunofluorescence |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Biochem/physiol Actions: | IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (Fragment antigen binding) fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Binds all rat Igs |
| General description: | IgG is present in large quantities in the human serum. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bon |
| Other Notes: | Antibody adsorbed with human IgG. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |