3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA
SIGMA/T4319 - peroxidase substrate
Synonym: TMB
Product Type: Chemical
Sigma offers specially formulated TMB liquid substrate systems for ELISA requiring a soluble end-product and for western blotting procedures that require an insoluble end-product. TMB Liquid Substrate System for peroxidase-based ELISA procedures where the soluble blue end product can be read at 370 nm or 655 nm. The addition of an acidic stopping solution produces a yellow color that can be read at 450 nm. The Sigma TMB Substrate System for Membranes produces an insoluble, dark blue end-product, and is not recommended for ELISA or immunohistochemical applications.
| form | liquid |
| Quality Level | 300  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Application: | 3,3′,5,5′-Tetramethylbenz |
| Application: | Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 25% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |