Turbonuclease from Serratia marcescens
SIGMA/T4330 - recombinant, expressed in E. coli
Synonym: Endonuclease from Serratia marcescens
CAS Number: 9025-65-4
MDL Number: MFCD00131010
Product Type: Chemical
Hydrolyzes double and single-stranded DNA and RNA yielding 5′-phosphomononucleotide and 5′-phosphooligonucleotide end-products.
| application(s) | life science and biopharma |
| biological source | Serratia marcescens |
| concentration | ≥200,000 units/mL |
| form | liquid |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| suitability | suitable for cell lysis |
| technique(s) | DNA purification: suitable |
| Application: | Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies. |
| Application: | Turbonuclease has been used in a study to assess the TY3 gag3 spacer effect on intracellular condensation and uncoating. |
| Application: | Used for the removal of nucleic acid from protein samples. |
| Biochem/physiol Actions: | Digests native or heat-denatured DNA and RNA. |
| Biochem/physiol Actions: | Endonuclease from Serratia marcescens is effective against both single- and double-stranded DNA and RNA. It mediates the digestion of the 3′ O—P bond resulting in oligonucleotides ending with 5′ monophosphate. The activity of this enzyme is known to be less affected by the reducing and chaotropic agents. It is highly stable at room temperature. This endonuclease eliminates the undesired nucleic acids in downstream processing. |
| Biochem/physiol Actions: | Turbonuclease provides a nuclease treatment by reducing viscosity and degrading RNA, genomic DNA, baculovirus DNA, and unencapsidated vector DNA. |
| General description: | Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases. |
| Physical form: | Supplied as a solution in 50 mM Tris-HCl, pH 8.0 and 50 mM NaCl |
| Unit Definition: | One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.30.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |