Tris-Borate-EDTA buffer
SIGMA/T4415 - BioReagent, suitable for electrophoresis, 10× concentrate
Synonym: TBE buffer
MDL Number: MFCD00236358
Product Type: Chemical
A representative image of the product packaging
| form | solution |
| impurities | DNase and RNase, none detected |
| InChI | 1S/C10H16N2O8.C4H11NO3.BH |
| InChI key | OSBLTNPMIGYQGY-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | OB(O)O.NC(CO)(CO)CO.OC(=O |
| suitability | suitable for electrophoresis |
| Application: | Ready for use in gel electrophoresis after dilution to working concentrations. Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE). |
| Application: | TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution. |
| General description: | TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water). |
| Other Notes: | TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance. |
| Packaging: | The 4L, 10L and 20L sizes are supplied in dispenser with a spigot. |
| Preparation Note: | Prepared with 18 megohm water |
| Preparation Note: | Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134). |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H360FD |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| Hazard Codes | T |
| Risk Statements | 60-61 |
| Safety Statements | 53-35-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41105319 |