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3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA

SIGMA/T4444 - ready to use solution

Synonym: TMB

CAS Number: 54827-17-7
MDL Number: MFCD00007748
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-T4444-100ML 100 mL
 $126.00
1/EA		 Add To Favorites
45-T4444-4X100ML 100 mL
 $379.00
4/EA		 Add To Favorites

 

form aqueous solution
InChI 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key UAIUNKRWKOVEES-UHFFFAOYSA-N
Quality Level 200 
shipped in wet ice
SMILES string Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
storage temp. 2-8°C
Application: 3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:
• to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectant
• to measure the levels of α-hemolysin toxin
• in imaging bound IgGs
Application: 3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
General description: This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352204

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