Thioglucosidase from Sinapis alba (white mustard) seed
SIGMA/T4528 - ≥100 units/g solid
Synonym: Glucosinolase; Myrosinase; Thioglucoside glucohydrolase
CAS Number: 9025-38-1
MDL Number: MFCD00132422
Product Type: Chemical
| biological source | plant seeds (Sinapis alba) |
| form | solid |
| Quality Level | 100  |
| specific activity | ≥100 units/g solid |
| storage temp. | −20°C |
| Application: | Thioglucosidase from Sinapis alba (white mustard) seeds has been used as a standard to quantify myrosinase activity and in column glucosinolate analysis of plant samples. |
| Application: | Thioglucosidase has been used in a study to assess Brassica species screening for glucosinolate content. Thioglucosidase has also been used in a study to investigate a negative regulatory role for auxin in sulphate deficiency response in Arabidopsis thaliana. |
| Biochem/physiol Actions: | Myrosinases are present in many bacteria, fungi, and edible plants, including those of the Brassicaceae (Cruciferae) family. The enzyme hydrolyzes the S-glucosidic bond of a glucosinolate substrate to form an unstable aglycone that rearranges with the loss of sulfate primarily to the isothiocyanate, though thiocyanates and nitriles are also formed. Many of the isothiocyanate products of aliphatic and aromatic glucosinolates have cancer chemopreventive properties. |
| Biochem/physiol Actions: | Thioglucosidase research has focused mainly on the cruciferous crops due to their economic importance and cancer preventive benefits. |
| General description: | Thioglucosidase, also called as myrosinase, is present in the myrosin cells that do not contain glucosinolates. This enzyme is obtained from several plant sources, such as Lepidium sativum, L. Sinapis alba and Brassica napus. |
| Unit Definition: | One unit will produce 1.0 μmole glucose per min from sinigrin at pH 6.0 at 25 °C. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥100 units/g solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.147 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |