Thrombin from bovine plasma
SIGMA/T4648 - lyophilized powder, 40-300 NIH units/mg protein (biuret)
Synonym: Factor IIa
CAS Number: 9002-04-4
EC Number: 232-648-7
MDL Number: MFCD00166053
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T4648-10KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: T4648-1KU
| biological source | bovine plasma |
| composition | Protein, 40-60% |
| form | lyophilized powder |
| mol wt | heavy chain ~33 kDa |
| light chain ~5 kDa | |
| Quality Level | 300  |
| shipped in | wet ice |
| specific activity | 40-300 NIH units/mg protein (biuret) |
| storage temp. | −20°C |
| UniProt accession no. | P00735  |
| Analysis Note: | Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K. |
| Analysis Note: | The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations. |
| Application: | Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess persistent hypocoagulability in patients with septic shock. |
| Biochem/physiol Actions: | Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B. |
| General description: | Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. |
| Other Notes: | View more information on thrombin at www.sigma-aldrich.com/enz. |
| Packaging: | 1000, 10000, 100000 units in glass bottle |
| Physical form: | Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0 |
| Preparation Note: | Activated with bovine brain thromboplastin. |
| Unit Definition: | Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 40-300 NIH units/mg protein (biuret) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.5 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |