Trizma® base
SIGMA/T4661 - ≥99.9% (titration), crystalline
Synonym: 2-
CAS Number: 77-86-1
Empirical Formula (Hill Notation): C4H11NO3
Molecular Weight: 121.14
EC Number: 201-064-4
MDL Number: MFCD00004679
Linear Formula: NH2C(CH2OH)3
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T4661-100G
This image depicts SKU: T4661-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: T4661-1KG
This image depicts SKU: T4661-1KG
| λ | (290nm < 0.05) |
| in 5 mM Tris; DNA (40% (w/w)) | |
| application(s) | diagnostic assay manufacturing |
| assay | ≥99.9% (titration) |
| bp | 219-220 °C/10 mmHg (lit.) |
| color | white |
| description | aminopeptidase substrate |
| form | crystalline |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7) |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA |
| loss | ≤5% (loss on drying, 110 °C) |
| mp | 167-172 °C (lit.) |
| pH | 10.0-11.5 |
| pKa (25 °C) | 8.1 |
| Quality Level | 400  |
| SMILES string | NC(CO)(CO)CO |
| solubility | water: soluble (400 g plus 600 mL of water) |
| useful pH range | 7-9 |
| Analysis Note: | Not pyrogen tested. Certificates of analysis are available with lot specific information. |
| Application: | Tris base has been used: • As a component of lysis buffer for cell disruption • In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis) • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols |
| General description: | Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight. |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 100 g in poly bottle |
| Packaging: | 5 kg in poly drum |
| Packaging: | Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.9% (titration) |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.) |
| UNSPSC | 12352104 |