Anti-TRPC3 antibody produced in rabbit
SIGMA/T5067 - affinity isolated antibody, lyophilized powder
Synonym: Anti-Transient receptor potential cation channel, subfamily C, member 3
MDL Number: MFCD02686005
Product Type: Chemical
Immunohistochemistry Anti-TRPC3: Cat. No. T5067: Immunohistochemical staining of Rat pituitary gland paraffin embedded sections using Anti-TRPC3 antibody, (1:100). TRPC3 is mainly expressed in the adenohypophysis (on left). Hematoxilin is used as the counterstain.
Immunohistochemistry Anti-TRPC3: Cat. No. T5067: Immunohistochemical staining of Mouse cerebellum using Anti-TRPC3 antibody (A). Immunoreactivity appears in the molecular layer and in Purkinje cells (B).
Immunohistochemistry Anti-TRPC3: Cat. No. T5067: Immunohistochemical staining of TRPC3 in Rat cerebellum using Anti-TRPC3 antibody. A. TRPC3 (green) appears in Purkinje cells (arrows) including both soma and dendrites and as well as in the molecular layer neuropil (asterisk). Staining of the same section with Mouse Anti-Parvalbumin (red) reveals that TRPC3 is not expressed in molecular layer interneurons. B. Pre-incubating Anti-TRPC3 with the TRPC3 peptide antigen blocks staining. DAPI is used as the counterstain (blue).
Immunohistochemistry Anti-TRPC3: Cat. No. T5067: Immunohistochemical staining of Rat cerebellum frozen section using Guinea Pig Anti-TRPC6 antibody and rabbit Anti-TRPC3 antibody. A. TRPC6 staining (green) appears in molecular layer and in Purkinje cells. B. In the same section, staining of TRPC3 (red) appears as well in both molecular layer and Purkinje cells. C. Merge images of A and B indicates extensive co-localization. DAPI is used as the counterstain (blue).
Immunocytochemistry Anti-TRPC3: Cat. No. T5067: Immunocytochemical staining of Paraformaldehyde-fixed and permeabilized Rat C6 brain glioma cells. A. Staining using Anti-TRPC3 antibody, (1:500) followed by Goat Anti-Rabbit-AlexaFluor-488 secondary antibody. B. Nuclear staining using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
Immunoblotting Anti-TRPC3: Cat. No. T5067: Western blot analysis of Rat heart membranes. Lanes 1. Anti-TRPC3 antibody, (1:200). 2. Anti-TRPC3 antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ≥0.6 mg/mL (after reconstitution) |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q9JMI9  |
| Biochem/physiol Actions: | TRPC3 (transient receptor potential-canonical 3) participates in motility and capacitation of sperm. It is responsible for the proliferation of ovarian cancer cell in vitro and in vivo. TRPC3 plays a key role in a wide spectrum of Ca2+ signaling mechanisms. Elevated expression of TRPC3 has been observed in spontaneously hypertensive rats (SHR). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Transient receptor potential-canonical 3 (TRPC3) is a member of TRP superfamily. It is mainly expressed in brain. The TRPC3 gene is mapped to human chromosome 4. |
| Immunogen: | peptide corresponding to amino acid residues 822-835 of mouse TRPC3. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 0.05% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |