Anti-β-Tubulin Isotype III antibody, Mouse monoclonal
SIGMA/T5076 - clone SDL.3D10, purified from hybridoma cell culture
Synonym: Anti-CDCBM; Anti-CDCBM1; Anti-CFEOM3; Anti-CFEOM3A; Anti-FEOM3; Anti-TUBB4; Anti-beta-4
MDL Number: MFCD08705576
Product Type: Chemical
Immunofluorescence Mouse cortical neurons labeled with Monoclonal Anti-β-Tubulin, Isotype III (Cat. No. T5076) (red) and anti-IL15 (green). From Diego Gomez Nicola, Functional and Systems Neurobiology, Cajal Institute (CSIC), Madrid, Spain.
Western Blotting Whole extract of Rat Brain were separated on SDS-PAGE and probed with Monoclonal Anti-β-Tubulin Isotype III antibody produced in Mouse, Clone: SDL.3D1 (Cat. No. T5076). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 0.2 μg/mL antibody 2. 0.1 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SDL.3D10, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human, boar, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.1-0.2 μg/mL using rat brain extract | |
| UniProt accession no. | Q13509  |
| Application: | Monoclonal anti-β-tubulin isotype III antibody (diluted 1:500) can be used as a primary antibody in immunocytochemical analysis of neurons. It can also be used for the localization of isotype III of β-tubulin by several immunochemical assays like dot blot, ELISA and immunohistochemistry. |
| Application: | Monoclonal Anti-β-Tubulin Isotype III antibody produced in mouse has been used in immunolabelling and flow cytometry. |
| Biochem/physiol Actions: | Tubulin β 3 class III (TUBB3) regulates neurogenesis and is involved in axon guidance and maintenance. Mutations in this gene is associated with hypoplasia of commissural axon (CA), congenital fibrosis of the extraocular muscles type 3 (CFEOM3) and dysgyria. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tubulin β 3 class III (TUBB3) encodes for the microtubule protein tubulin β‐3, which is majorly expressed in neurons. The gene is located on human chromosome 16q24.3. |
| General description: | Tubulin is the main constituent of microtubules made up of two similar polypeptides α and β, each with 50 kD (approx.) molecular weight. It has several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin isotype III antibody can be used in microarray and western blotting. It can also be used in immunoblotting. Mouse anti-β-tubulin isotype III antibody reacts specifically with an epitope present on the β-tubulin (isotype III) of bovine, rat, mouse, boar and human but not with tubulin present in chicken brain preparation or in a chicken fibroblasts cell line. |
| Immunogen: | peptide corresponding to the carboxy-terminal sequence of human β-tubulin isotype III, conjugated to BSA. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |