Monoclonal Anti-α-Tubulin antibody produced in mouse
SIGMA/T5168 - clone B-5-1-2, ascites fluid
Synonym: Alpha Tubulin Antibody Sigma; Anti Tubulin Sigma; Tubulin Antibody Sigma; Tubulin Antibody Sigma - Monoclonal Anti-α-Tubulin antibody produced in mouse
MDL Number: MFCD00145891
Product Type: Chemical
Western Blotting JOURNAL CITATION: Poly(I:C) induces intense expression of c-IAP2 and cooperates with an IAP inhibitor in induction of apoptosis in cancer cells. By: Friboulet, L., Gourzones, C., et al. in BMC Cancer, 2010. PubMed ID: 20576118 Image collected and cropped by CiteAb from the following publication, (http://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-10-327), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Poly(I:C) induces intense expression of c-IAP2 and cooperates with an IAP inhibitor in induction of apoptosis in cancer cells. By: Friboulet, L., Gourzones, C., et al. in BMC Cancer, 2010. PubMed ID: 20576118 Image collected and cropped by CiteAb from the following publication, (http://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-10-327), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: The Caenorhabditis elegans Elongator complex regulates neuronal alpha-tubulin acetylation. By: Solinger, J. A., Paolinelli, R., et al. in PLoS Genet, 2010. PubMed ID: 20107598 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1000820), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Human DAOY cells were stained using Monoclonal Anti-α-Tubulin, clone no. B-5-1-2 (Cat. No. T5168) with Anti-Mouse-FITC (green) and Hoechst 33528 (blue). From Yuying Jiang, Columbus Children’s Research Institute, Columbus, OH.
Enhanced Validation-By Independent Antibodies Chicken fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Monoclonal Anti-α-Tubulin antibody produced in Mouse, Clone: DM1A (Cat. No. T9026) (A). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262), and 1 μg/mL Monoclonal Anti-α-Tubulin antibody produced in Mouse, Clone: B-5-1-2 (Cat. No. T5168) (B). The antibody was developed using 1:40 Goat Anti-Mouse IgG (Fab specific)-FITC antibody (Cat. No. F5262). Results Two Anti-α-Tubulin antibodies, T9026 (A) and T5168 (B) target different regions of α-Tubulin show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human appendix sections with 1:1,000 Monoclonal Anti-α-Tubulin Clone: B-5-1-2 (Cat. No. T5168) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1:4,000 Monoclonal Anti-α-Tubulin Clone: B-5-1-2 (Cat. No. T5168). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. PC-12 6. RAT2 7. CHO 8. MDBK 9. MDCK
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | B-5-1-2, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| enhanced validation | independent ( Antibodies) Learn more about Antibody Enhanced Validation  |
| isotype | IgG1 |
| mol wt | antigen ~50 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:2,000 using cultured human or chicken fibroblasts |
| radioimmunoassay: suitable | |
| western blot: 1:4,000 using human fibroblast cell extract | |
| UniProt accession no. | P68366  |
| Application: | Monoclonal Anti-α-Tubulin antibody produced in mouse has been used: • In immunofluorescence Analysis • In western blotting/ Immunoblotting • For immunolabelling cells in electron microscopy |
| Biochem/physiol Actions: | Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS). α-Tubulin is a key regulator of cytoskeletal proteins. It mediates cellular developmental stages such as proliferation, migration, signalling and also maintains the shape of the cell. α-Tubulin controls trafficking, signaling and cellular tensegrity mediated by microtubules. The encoded protein is associated with the development and progression of cancer. α-Tubulin acetylation potentiates the metastatic property of breast cancer. Mutation in TUBA4A is associated with the development of various types of cancers, such as oral cancer, breast cancer, rectal cancer, lung cancer and prostate cancer. In addition, variation in the TUBA4A leads to sporadic amyotrophic lateral sclerosis (ALS). Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and the cytoskeleton. |
| Biochem/physiol Actions: | Tubulin α mediates the formation of cytoskeletal protein polymers. It is necessary for cell cycle and controls cellular movement and signaling. Tubulin α is known to maintain the shape of the cells. It is an active part of oncogenesis and progression of different types of cancer. Posttranslationally modified Tubulin α governs microtubule functions including cell trafficking and cellular tensegrity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the B-5-1-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin is the major building block of microtubules. |
| General description: | The TUBA4A (tubulin α 4a), is mapped to human chromosome 2q35. The gene codes for a member of the a -tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. The α-subunit of tubulin has molecular weight of 50,000. |
| Immunogen: | Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Liquid |
| Specificity: | Monoclonal Anti-alpha-Tubulin antibody recognizes an epitope at the C-terminal end of the α-tubulin isoform in various organisms. |
| Specificity: | Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |